Angiotensin-converting enzyme 2 (ACE2); a first homolog of ACE; regulates the renin angiotensin system (RAS) by counterbalancing ACE activity. Accumulating evidence in recent years has demonstrated a physiological and pathological role of ACE2 in the cardiovascular; renal and respiratory systems. ACE2 also has an important role in blood pressure control. This enzyme; an homolog of ACE; hydrolyzes angiotensin (Ang) I to produce Ang-(1-9); which is subsequently converted into Ang-(1-7) by a neutral endopeptidase and ACE. ACE2 releases Ang-(1-7) more efficiently than its catalysis of Ang-(1-9) by cleavage of Pro(7)-Phe(8) bound in Ang II. Thus; the major biologically active product of ACE2 is Ang-(1-7); which is considered to be a beneficial peptide of the RAS cascade in the cardiovascular system. A physiological role for ACE2 has been implicated in hypertension; cardiac function; heart function and diabetes; and as a receptor of the severe acute respiratory syndrome coronavirus. In the acute respiratory distress syndrome (ARDS); ACE; AngII; and AT1R promote the disease pathogenesis; whereas ACE2 and the AT2R protect from ARDS. Importantly; ACE2 has been identified as a key SARS-coronavirus receptor and plays a protective role in severe acute respiratory syndrome (SARS) pathogenesis. Furthermore; the recent explosion of research into the ACE2 homolog; collectrin; has revealed a new physiological function of ACE2 as an amino acid transporter; which explains the pathogenic role of gene mutations in Hartnup disorder. This review summarizes and discusses the recently unveiled roles for ACE2 in disease pathogenesis.
A DNA sequence encoding the human ACE2 (NP_068576.1) (Met1-Ser740) was expressed with the Fc region of mouse IgG1 at the C-terminus.
> 90 % as determined by reducing SDS-PAGE.
Lyophilized from sterile PBS; pH 7.4
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.