Custom Recombinant Protein Service

Please fill in the form below and a sales specialist will contact you with a quotation as soon as possible.

Basic Information
	Gene/Protein Name:
	Species:
	Molecular Weight:
	GenBank Accession Number:
	Nucleotide Sequence:
	Amino Acid Sequence (please include mutation sequence if applicable):

Protein Applications
		Activity Assay		Drug Screening		Cell-based Assay
		Structural Study		HTS		Activity detection
		NMR		X-ray crystallisation		Use as antigen
		In vivo activity study		Protein-protein interaction		Other

Previous Experimental Data and Results
	Expression System:
	Expression Vector:
	Expression Conditions:
	Expression Results:
	Please describe any previous attempts to express and purify target protein:

Gene Information
		Gene Synthesis		Gene Synthesis (Including codon optimization)
		Client offers expression plasmid		Client owill provide non-expressing DNA plasmid (subcloning required)
		Plasmid selected from Assay Genie cDNA library		Other

Expression Vector
		Assay Genie uses proprietary expression vector (if applicable)		Customer provides ready-to-use expression vector
		Client specifies expression vector

Expression System
		E.coli expression		Mammalian expression
		Baculovirus expression		Yeast expression
		Insect cell expression		No specific requirement

Tag
		N-His		N-His-sumo		N-GST
		N-MBP		N-Flag		C-His
		C-Flag		N-HA		N-Myc
		C-HA		C-Myc		No tag

Tag Removal Required?		Yes	No

Buffer
		PBS dissolution		dH2O dissolution
		Glycerinobactam		No specific requirement
		Other

Purification Method	Performed by Assay Genie	Performed by client

Deliverable Requirements
	Protein Quantity (mg):
	Protein Purity (%):
	Protein Concentration:
	Endotoxin level:
	Aliquot requirements (ug/tube):

Protein Form (choose one)	Soluble	Insoluble or Inclusion Body form

Protein Refolding
Protein Refolding	If the protein is expressed as inclusion bodies, is protein refolding desired?	Yes	No

Protein Validation Methods		SDS-PAGE		Western Blot		Activity Detection

Grant Status		The proposal will be used to apply for a grant

Other Notes:

Note: Please ensure all fields marked * are complete.