Recombinant Proteins for SARS-CoV-2 Research

Assay Genie SARS-CoV-2 Recombinant Proteins

With the rapid progression of the global pandemic, COVID-19, there has been the development of innovative drug & vaccine RnD strategies to battle and overcome this infectious disease. At Assay Genie we have developed over 60  novel recombinant proteins for SARS-CoV-2 as well as new variants such as Japan, UK, South Africa, Brazil and others to aid COVID-19 Research.

Why choose Assay Genie SARS-CoV-2 recombinant proteins?

Our proteins are highly pure, ranging between 90-98% in purity. They are also physiologically relevant; Assay Genie recombinant proteins are mostly expressed in mammalian expression systems, meaning that they are synthesized, processed, and secreted in a manner similar to in humans. For this reason, as well as the fact they have been shown to have low endotoxin, our recombinant proteins do not elicit an immune response in vivo. This makes ELISA Genie recombinant proteins hugely relevant to the area of vaccine development for COVID-19.

Types of SARS-CoV-2 recombinant proteins available

We have synthesized recombinant versions of the key proteins involved in the process of viral infection by COVID-19. These include the integral proteins expressed on the surface of the coronavirus; spike (S) protein, envelope (E) protein, and nucleocapsid protein (N).We have also created recombinant proteins of other proteins involved in the mechanism of infection with coronavirus. These include proteinases, proteases, and helicases essential for coronaviral replication and host cell invasion, as well as human proteins implicated in coronavirus uptake into host cells, such as CD147/Basigin Protein, APN Protein, and the human receptor for SARS-CoV-2; ACE2.
Our recombinant proteins allow scientists to both recapitulate the endogenous activity of key proteins involved in coronavirus infection, as well as develop potential therapies by targeting such proteins.

SARS-CoV-2 recombinant proteins

Sku Product Name Conjugation Region Virus
CARP01258 Recombinant SARS-CoV-2 Spike RBD Protein His Tag Arg319-Phe541 SARS-CoV-2
CARP01259 Recombinant SARS-CoV-2 Spike S1 Protein His Tag Val11-Arg682 SARS-CoV-2
CARP01260 Recombinant SARS-CoV-2 Spike S1+S2 ECD Protein His Tag Val11-Gln1208 SARS-CoV-2
CARP01261 Recombinant SARS-CoV-2 Spike S1 Protein His Tag Gln14-Arg683 SARS-CoV-2
CARP01262 Recombinant SARS-CoV-2 Spike (S) S1 Protein His Tag Val16-Arg685 SARS-Cov-2
CARP01263 Recombinant SARS-CoV-2 Envelope (E) Protein His Tag Met1-Val75 SARS-Cov-2
CARP01264 Recombinant SARS-CoV-2 Nucleocapsid (N) Protein His Tag Met1-Ala419 SARS-Cov-2
RPES0001 Recombinant SARS-CoV-2 3C-like Proteinase Protein His Tag Ser1-Gln306 SARS-Cov-2
RPES0002 Recombinant SARS-CoV-2 Helicase Protein His Tag Ala1-Gln601 SARS-Cov-2
RPES0003 Recombinant SARS-CoV-2 NSP1 Protein His Tag Met1-Gly180 SARS-Cov-2
RPES0004 Recombinant SARS-CoV-2 NSP1 Protein His Tag Ala1-Gly638 SARS-Cov-2
RPES0005 Recombinant SARS-CoV-2 NSP7 Protein His Tag Ser1-Gln83 SARS-Cov-2
RPES0006 Recombinant SARS-CoV-2 NSP8 Protein G476S Arg319-Phe541 SARS-Cov-2
RPES0007 Recombinant SARS-CoV-2 NSP10 Protein His Tag Ala1-Gln139 SARS-Cov-2
RPES0008 Recombinant SARS-Cov-2 Papain-Like Protease Protein His Tag Glu1564-Lys1878 SARS-Cov-2
RPES0010 Guanine-N7_meth Protein SARS-Cov-2 mFc Tag Asn331-Val524 SARS-Cov-2
RPES0011 Recombinant SARS-CoV-2 Spike Protein His Tag Asn331-Val524 SARS-Cov-2
RPES0012 Recombinant SARS-CoV-2 Spike Protein mFc Tag Arg319-Pro589 SARS-Cov-2
RPES0013 Recombinant SARS-CoV-2 Spike Protein His Tag Arg319-Ser591 SARS-Cov-2
RPES0014 Recombinant SARS-CoV-2 Spike Protein, Biotinylated Avi-His Tag Arg319-Ser591 SARS-Cov-2
RPES0015 Recombinant SARS-CoV-2 Spike S1 Protein mFc Tag Gln14-Arg685 SARS-Cov-2
RPES0016 Recombinant SARS-CoV-2 Spike S1 Protein Fc Tag Gln14-Arg685 SARS-Cov-2
RPES0017 Recombinant SARS-CoV-2 Spike S1 Protein Gln14-Arg685 SARS-Cov-2
RPES0018 Recombinant SARS-CoV-2 Spike S1 Protein His Tag Val16-Arg685 SARS-Cov-2
RPES0019 Recombinant SARS-CoV-2 Spike S1 Protein His Tag SARS-Cov-2
RPES0020 Recombinant SARS-CoV-2 Spike Protein His Tag SARS-Cov-2
RPES0021 Recombinant SARS-CoV-2 Nucleocapsid Protein His Tag Met1-Ala419 SARS-Cov-2
RPES0022 Recombinant SARS-CoV-2 Nucleoprotein NTD domain His Tag SARS-Cov-2
RPES0023 Recombinant SARS-CoV-2 Nucleoprotein CTD domain Protein SARS-Cov-2
RPES0024 Recombinant SARS-CoV-2 Envelope Protein His Tag Met1-Val75 SARS-Cov-2
RPES0025 Recombinant SARS-CoV-2 S-trimer Protein His Tag SARS-Cov-2
RPES0026 Recombinant SARS-CoV-2 Spike S2 Protein Fc Tag SARS-Cov-2
RPES0027 Recombinant Human ACE2 Protein His Tag Gln18-Ser740 SARS-Cov-2
RPES0028 Recombinant Human ACE2 Protein Fc Tag Gln18-Ser740 SARS-Cov-2
RPES0029 Recombinant Human ACE2 Protein Avi-His Tag Gln18-Ser740 SARS-Cov-2
RPES0030 Recombinant Human ACE2 Protein mFc Tag Gln18-Ser740 SARS-Cov-2
RPES0031 Recombinant Human CD147/Basigin Protein His Tag Ala22-His205 SARS-Cov-2
RPES0032 Recombinant Human CD147/Basigin Protein His Tag Ala22-Arg325 SARS-Cov-2
RPES0033 Recombinant Human Cathepsin B/CTSB Protein His Tag Arg18-Ile339 SARS-Cov-2
RPES0034 Recombinant Mouse Cathepsin B/CTSB Protein His Tag Met 1-Phe 339 SARS-Cov-2
RPES0035 Recombinant Human Cathepsin L/CTSL Protein His Tag Thr18-Val333 SARS-Cov-2
RPES0036 Recombinant Mouse Cathepsin L/CTSL Protein His Tag Thr18-Asn334 SARS-Cov-2
RPES0037 Recombinant Mouse Cathepsin L/CTSL Protein His Tag Lys69­Lys967 SARS-Cov-2
RPES0038 Recombinant Mouse APN Protein His Tag Lys69­Ser966 SARS-Cov-2
RPES0039 Recombinant SARS-CoV-2 Nucleocapsid Protein His Tag Met1-Ala419 SARS-Cov-2
RPES0040 Recombinant SARS-CoV-2 Spike S1+S2 Protein His Tag Val16-Pro1213 SARS-Cov-2
RPES0041 Recombinant SARS-CoV-2 Spike Protein His Tag Arg319-Phe541 SARS-Cov-2
RPES0042 Recombinant SARS-CoV-2 Spike Protein mFc Tag Arg319-Phe541 SARS-Cov-2
RPES0043 Recombinant SARS-CoV-2 Spike Protein, Biotinylated His Tag Arg319-Phe541 SARS-Cov-2
RPES0044 Recombinant SARS-CoV-2 Spike S1 Protein Fc Tag Val16-Arg685 SARS-Cov-2
RPES0045 Recombinant SARS-CoV-2 Spike S1 Protein His Tag Val16-Arg685 SARS-Cov-2
RPES0046 Recombinant SARS-CoV-2 Spike S1 Protein mFc Tag Val16-Arg685 SARS-Cov-2
RPES0047 Recombinant SARS-Cov-2 Spike S2 Protein His Tag Ser686-Pro1213 SARS-Cov-2
RPES0048 Recombinant SARS-CoV-2 Nucleoprotein Protein His Tag Met1-Ala422 SARS-Cov-2
RPES0049 Recombinant SARS-CoV-2 Spike RBD Protein His Tag Arg306-Phe527 SARS-Cov-2
RPES0050 Recombinant Human ACE2 Protein His Tag Met1-Ser740 SARS-Cov-2
RPES0051 Recombinant SARS-CoV-2 Papain-like Protease Protein His Tag Glu1564-Val1880 SARS-Cov-2

Variant SARS-CoV-2 recombinant proteins available

Sku Product Name Variant Virus
DC0007150 Recombinant SARS-CoV-2 Spike RBD Variant 501Y.V1 United Kingdom SARS-CoV-2
DC0007151 Recombinant SARS-CoV-2 Spike RBD Variant 501Y.V2 South Africa SARS-CoV-2
DC0007152 Recombinant SARS-CoV-2 Spike RBD Variant 501Y.V3 Brazil & Japan SARS-CoV-2
PACOV0016 Recombinant Novel Coronavirus Spike glycoprotein(S) (Active) E484K SARS-CoV-2
RPES0068 Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag) V367F SARS-Cov-2
RPES0069 Recombinant 2019-nCoV Spike Protein (RBD, His Tag) V367F SARS-Cov-2
RPES0070 Recombinant 2019-nCoV Spike Protein (RBD, His Tag) K458R SARS-Cov-2
RPES0071 Recombinant 2019-nCoV Spike Protein (RBD, His Tag) F342 SARS-Cov-2
RPES0072 Recombinant 2019-nCoV Spike Protein (RBD, His Tag) V483A SARS-Cov-2
RPES0073 Recombinant 2019-nCoV Spike Protein (RBD, His Tag) A435S SARS-Cov-2
RPES0074 Recombinant 2019-nCoV Spike Protein (RBD, His Tag) N354D SARS-Cov-2
RPES0075 Recombinant 2019-nCoV S1 Protein (His Tag) (Active) D614G SARS-Cov-2
RPES0077 Recombinant 2019-nCoV Spike Protein (RBD, His Tag) (Active) G476S SARS-Cov-2
RPES0078 Recombinant 2019-nCoV Spike Protein (RBD, His Tag) (Active) W436R SARS-Cov-2

COVID-19 Background: SARS-CoV-2 Structure

SARS-CoV-2 is the causative viral agent of the disease COVID-19. It bears several key proteins within its structure which have been the focus of research the past number of months due to their potential to be therapeutic targets in COVID-19.

Key SARS-CoV-2 Proteins

Spike (S) Protein

The spike protein is the one of the four main proteins found on the surface of the coronavirus virion. These transmembrane glycoprotein spikes consists of two components; S1 and S2.  S1 and S2 proteins have been shown to play a major role in receptor binding and membrane fusion. The receptor-binding domain (RBD) of the S protein can be found at the N-Terminal end of the S1 domain. It is responsible for host cell entry of the virus via the ACE2 receptor. Upon binding of the Spike S1 domain to ACE2 a conformational change is initiated in the Spike S2 domain; this facilitates the attachment of the virus to the host cell membrane which results in subsequent nucleocapsid transfer into cell.

ACE2 Protein

Angiotensin II converting enzyme (ACE2), a carboxypeptidase, is an enzyme involved in the renin-angiotensin system (RAS). It has been shown to play an important role in regulating the heart function. ACE2 is a receptor of human coronaviruses, such as SARS and HCoV-NL63. This receptor facilitates the uptake of SARS-CoV-2 into host cells. ACE2 is expressed in multiple locations and cell types, notably including alveolar epithelial cells, surface enterocytes within the small intestine, and vascular endothelial cells of the lung, kidneys and heart.

Nucleocapsid (N) Protein

Coronavirus N protein is the most abundant protein expressed on the surface of the coronavirus. It is required for coronavirus RNA synthesis and packaging, and has RNA chaperone activity that may be involved in template switch. The N  has also been suggested to play a role in virus particle release.

Envelope (E) Protein

The envelope protein is one of the four integral proteins found on SARS-CoV-2 viral particles. The protein has a central role in virus morphogenesis and assembly, acting as a viroporin and self-assembling in host membranes to form pentameric protein-lipid pores that allow ion transport. The E protein also plays a role in the induction of apoptosis (by similarity).