Recombinant SARS-Cov-2 Papain-Like Protease Protein (RPES0008)

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SKU:
RPES0008
€300

Description

Information Description
Background:
Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.
Size:

10µg

Product Name:
SARS-Cov-2 Papain-Like Protease Protein
Activity:
Synonym:
Papain-like Protease; PLpro; PL-PRO; pp1a; Replicase polyprotein 1a
Protein Construction:
SARS-Cov-2 Papain-like Protease is produced by our E.coli expression system and the target gene encoding Glu1564-Lys1878 is expressed.
Sequence:
Glu1564-Lys1878
Fusion tag:
Accession:
QHD43415.1
Species:
virus
Expressed Host:
E.coli
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Endotoxin:
< 1.0 EU per µg as determined by the LAL method.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Mol Mass:
35.8 kDa
AP Mol Mass:
34 kDa
Formulation:
Supplied as a 0.2 uM filtered solution of 20mM Tris-HCl, 10 mM Mercaptoethanol [BME], 20% Glycerol, pH 7.5.
Reconstitution:
Please refer to the printed manual for detailed information.
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