Recombinant SARS-CoV-2 Spike S1+S2 ECD Protein

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Recombinant Protein
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Technical Manual

SARS-CoV-2 Spike Protein - Background Information

Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc.. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS).

The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

SARS-CoV-2 Spike S1 +S2 ECD Protein Properties

Description Recombinant 2019-nCoV S1+S2 ECD (S-ECD) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Gln1208) of 2019-ncov S1+S2 ECD (S-ECD) (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.
Size 100µg
Expressed Host HEK293 cells
Species SARS-CoV-2
Application Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation
Purity >97% by SDS-PAGE.
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Reconstitution Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
Gene ID 43740568
Endotoxin < 0.1 EU/μg of the protein by LAL method.
Storage This product is stable at ≤ ‑70° C for up to 6 months from the date of receipt.
For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
Tag 6×His tag at the C-terminus.