Recombinant Human PGD Protein (Human Cells, His Tag) (RPES4005)



ELISA Kit Technical Manual

Recombinant Human PGD Protein (Human Cells, His Tag)

Background:6-phosphogluconate dehydrogenase(PGD) is a cytoplasm-located protein, and belongs to the 6-phosphogluconate dehydrogenase family. 6PGD is the second dehydrogenase in the pentose phosphate shunt. It catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH. Mutations within the gene coding this enzyme result in 6-phosphogluconate dehydrogenase deficiency, an autosomal hereditary disease effecting the red blood cells.
Name:Recombinant Human PGD Protein (Human Cells, His Tag)
Assay Genie SKU:RPES4005
Synonyms:6-phosphogluconate dehydrogenase; Decarboxylating;PGD;PGDH;6PGD
Expression host:Human Cells
Sequence:Met 1-Ala483
Mol Mass:54.2 kDa
AP Mol Mass:58 kDa
Purity:> 90 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Formulation:Supplied as a 0.2 um filtered solution of 20mM PB,150mM NaCl,pH7.4.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:PGD: an oxidative carboxylase that catalyses the decarboxylating reduction of 6-phosphogluconate into ribulose 5-phosphate in the presence of NADP. This reaction is a component of the hexose mono-phosphate shunt and pentose phosphate pathways.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - pentose phosphate pathway; EC; Oxidoreductase; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: cytosol; nucleus

Molecular Function:phosphogluconate dehydrogenase (decarboxylating) activity; NADP binding

Biological Process: pentose-phosphate shunt; pentose-phosphate shunt, oxidative branch; carbohydrate metabolic process; pathogenesis; D-gluconate metabolic process; pentose biosynthetic process

NCBI Summary:6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]
UniProt Code:P52209
NCBI GenInfo Identifier:40068518
NCBI Gene ID:5226
NCBI Accession:NP_002622.2
UniProt Secondary Accession:P52209,Q9BWD8, A8K2Y9, B4DQJ8,
UniProt Related Accession:P52209
Molecular Weight:51,872 Da
NCBI Full Name:6-phosphogluconate dehydrogenase, decarboxylating isoform 1
NCBI Synonym Full Names:phosphogluconate dehydrogenase
NCBI Official Symbol:PGD
NCBI Official Synonym Symbols:6PGD
NCBI Protein Information:6-phosphogluconate dehydrogenase, decarboxylating
UniProt Protein Name:6-phosphogluconate dehydrogenase, decarboxylating
UniProt Gene Name:PGD
UniProt Entry Name:6PGD_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
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