10242 results for 'tnf best'

TUNEL Assay Kits | 1-Step In Situ Apoptosis Detection | Assay Genie

Leukemia Inhibitory Factor and JAK-STAT Signalling Pathway Leukemia inhibitory factor (LIF) is a cytokine that belongs to the interleukin-6 (IL-6) family of cytokines. Other members of

Fibroblasts, essential components of connective tissues, play vital roles in maintaining tissue structure and function. Their remarkable ability to produce and organize the extracellular matrix

Annexin V Apoptosis Assay Kits | Flow Cytometry Detection | Assay Genie

Immunology · Antibody ProductionHybridoma Technology: How Monoclonal Antibodies Are MadeHybridoma technology, pioneered by Georges Köhler and César Milstein in 1975, is the method that made monoclonal

Quick Facts About AvizakimabWhat is Avizakimab?Avizakimab is a monoclonal antibody designed to target the CD47 protein, a key checkpoint in immune regulation, particularly in cancer ther

Protein Analysis · ProtocolsWestern Blot Sample Preparation: A Practical GuideMost failed Western blots fail before the gel is loaded. Lysis buffer choice, inhibitor selection, accurate protein quanti

Oesophageal Adenocarcinoma is on the rise globally affecting more than 450,000 people worldwide (Pennathur, Gibson et al. 2013). Once diagnosed overall 5-year survival ranges from 15 % to 2

Cancer immunotherapy has revolutionized treatment options, offering strategies to harness the immune system to target and destroy tumors. One emerging area of interest is the use of OX40

Immunology · NeuroscienceHistamine Receptors: H1, H2, H3 & H4 ExplainedHistamine receptors are the G protein-coupled receptors through which histamine controls allergy, gastric acid secretion, neurotr

Quick Facts About TigatuzumabWhat is Tigatuzumab?Tigatuzumab is a monoclonal antibody that targets the death receptor 5 (DR5) pathway, inducing apoptosis in cancer cells.What is the mech

For decades, the fight against cancer has been a relentless battle of attrition, often with treatments that harm the body as much as the disease. But a new era of precision medicine is dawning, one th

Gastroenterology · Barrier BiologyZonulin and Intestinal PermeabilityZonulin is the best-characterised endogenous regulator of intestinal tight junctions — the protein that opens the gut barrier on de

Imagine a world where cancer treatment is no longer a blunt instrument but a precision-guided surgical strike, executed by the body's own immune system. For years, we have dreamed of 'smart' cells th

By Sophie Arthur How many diseases and conditions do you think exist in this world? Thousands? Hundreds of thousands? Maybe even millions? Unfortunately, I don’t know the answer. But what I

If you've ever had a urinary tract infection, you know all about bacteria. These tiny organisms can invade your body and cause all sorts of problems. But what you may not know is tha

Tertiary Lymphoid Structures: The Body's Hidden Arsenals in the Fight Against Cancer Imagine a battlefield within the body, where the immune system stands as the last line of defense against the rele

3D Organoid Cell Viability Assay (AKES081) effectively measures BENO model viability across different storage conditions. AKES081 detects significant viability differences, particularly

For decades, the primary strategy in cancer treatment has been to trigger apoptosis, a form of programmed cell death, in malignant cells. However, many cancers develop resistance to apoptosis-inducin

I'll start this post off with a story. When I was half way through my PhD and I had found my passion for science communication, I approached my supervisor for the first time to ask “Can I ha

Cell Signalling · Kinase CascadesMAPK Signaling: The Pathway of Mitogen StimulationThe mitogen-activated protein kinase (MAPK) pathway is one of the most important signalling cascades in biology, conv

By Shane Houston, PhD Candidate Queen’s University Belfast Cancer is an age old adversary of the human race. This out-of-control growth of abnormal cells has been a shadow over human health t

During my PhD I knew how networking was important, it helped me source reagents off other labs, get the best advice and helped me land a post doc at Cambridge University. Its not what y

Techniques · Gene DeliveryTransduction vs Transfection: Understanding Gene Delivery TechniquesTransduction and transfection are the two main routes for introducing genetic material into cells. Transdu

Why do powerful cancer immunotherapies work wonders for some patients but fail for others? The answer may lie not just in the cancer cells themselves, but in the energy levels of our own immune warrio

Cancer Biology · GeneticsTumour Suppressor Genes: Roles, Examples & CancerTumour suppressor genes are the brakes on cell growth — they restrain proliferation, repair damaged DNA and trigger apoptosis

The MycoGenie Rapid Mycoplasma Detection Kit enables the detection of 28 Mycoplasma species in under 1 hour using a simple visual determination method, eliminating the need for expensive

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer The five main types of ELISA are sandwich, competitive, direct, indirect and multiplex. Sandwich ELISA is

In 2006, upon completion of the Human Genome Project, it was discovered that nearly 8% of the human genome is made up of viral DNA. These viral remnants are composed of ancient germl

IntroductionThe human kinome is estimated to contain 518 genes, in comparison to the estimated 180 genes that comprise the phosphatome (Arena et al., 2005). These kinase genes represent a signif

Western Blot Protocol & Troubleshooting Guide | 14 Steps + 101 Tips | Assay Genie

Cell Biology · Protein DegradationThe Autophagy Pathway: Mechanism, Regulation and How to Measure ItAutophagy is the cell’s route for degrading and recycling its own contents — damaged organelles, agg

Explore 29 essential tips for crafting a compelling and well-structured PhD thesis, blending effective writing practices with time management and academic rigor for a successful dissertation journ

Cancer Biology · Cell Signallingp53: Structure, Signalling, Regulation and Mutation in Cancerp53 is the most frequently mutated gene in human cancer, and the reason is structural: a single tetrameric

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~10 min read Quick Answer A sandwich ELISA uses two antibodies — a capture ant

Cell Culture · ProtocolsHaemocytometer Cell Counting: Procedure, Calculation and Common ErrorsA haemocytometer turns a known chamber volume into a cell concentration, and the whole method rests on fou

Mononuclear phagocytes, essential in immunity and homeostasis, perform diverse roles from phagocytosis to tissue repair, influencing health and disease. Key Takeaways: Mononuclear p

Neuroscience · BiomarkersA Guide to Tau Proteins & TauopathiesTau keeps the neuronal cytoskeleton standing up. When its phosphorylation goes wrong, the same protein becomes the aggregate that defines

Immunology · Antibody BiologyAntibody Light Chains, Heavy Chains, Constant Regions & Tumour-Associated AntigensAn antibody’s specificity comes from its variable domains and its biological effect comes

Molecular Biology · ProtocolsTypical Workflow of CRISPR-Cas9 Genome EditingA CRISPR experiment is only as good as the steps around the cut. This guide walks the full workflow — designing and scoring a