Anti-VDAC1/Porin Antibody (CAB15735)

Antibody Name:	Anti-VDAC1 / Porin Antibody
Antibody SKU:	CAB15735
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human VDAC1 / Porin (NP_003365.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, A-549, A-431, Raji, Rat heart, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human VDAC1 / Porin (NP_003365.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAVP PTYA DLGK SARD VFTK GYGF GLIK LDLK TKSE NGLE FTSS GSAN TETT KVTG SLET KYRW TEYG LTFT EKWN TDNT LGTE ITVE DQLA RGLK LTFD SSFS PNTG KKNA KIKT GYKR EHIN LGCD MDFD IAGP SIRG ALVL GYEG WLAG YQMN FETA KSRV TQSN FAVG YKTD EFQL HTNV NDGT EFGG SIYQ KVNK KLET AVNL AWTA GNSN TRFG IAAK YQID PDAC FSAK VNNS SLIG LGYT QTLK PGIK LTLS ALLD GKNV NAGG HKLG LGLE FQA
Gene ID:	7416
Uniprot:	P21796
Cellular Location:	Cell membrane, Membrane raft, Mitochondrion outer membrane, Multi-pass membrane protein
Calculated MW:	30kDa
Observed MW:	36kDa

Synonyms:

PORIN, VDAC-1, VDAC1, VDAC1 / Porin

Background:

This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.

UniProt Protein Function:	VDAC1: a protein of the eukaryotic mitochondrial porin family. Forms a voltage-dependent anion channel through the mitochondrial outer membrane and also the plasma membrane. The channel allows diffusion of small hydrophilic molecules; it adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
UniProt Protein Details:	Protein type:Membrane protein, integral; Channel, misc.; Mitochondrial Chromosomal Location of Human Ortholog: 5q31 Cellular Component: lipid raft; membrane; mitochondrial outer membrane; mitochondrion; myelin sheath; nucleus; plasma membrane; pore complex; synaptic vesicle Molecular Function:porin activity; protein binding; protein complex binding; protein kinase binding; voltage-gated anion channel activity Biological Process: anion transport; apoptosis; behavioral fear response; epithelial cell differentiation; learning; macroautophagy; macromitophagy; mitochondrial calcium ion transport; nerve-nerve synaptic transmission; viral reproduction
NCBI Summary:	This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.[provided by RefSeq, Sep 2010]
UniProt Code:	P21796
NCBI GenInfo Identifier:	130683
NCBI Gene ID:	7416
NCBI Accession:	P21796.2
UniProt Secondary Accession:	P21796,Q5FVE7, Q9UIQ5, Q9UPL0, B3KVK4, D3DQ93,
UniProt Related Accession:	P21796
Molecular Weight:	30,773 Da
NCBI Full Name:	Voltage-dependent anion-selective channel protein 1
NCBI Synonym Full Names:	voltage dependent anion channel 1
NCBI Official Symbol:	VDAC1
NCBI Official Synonym Symbols:	PORIN; VDAC-1
NCBI Protein Information:	voltage-dependent anion-selective channel protein 1
UniProt Protein Name:	Voltage-dependent anion-selective channel protein 1
UniProt Synonym Protein Names:	Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31HL; Porin 31HM
Protein Family:	Porin
UniProt Gene Name:	VDAC1
UniProt Entry Name:	VDAC1_HUMAN

	Western blot analysis of extracts of various cell lines, using VDAC1 / Porin antibody (CAB15735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat heart using VDAC1 / Porin antibody (CAB15735) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using VDAC1 / Porin antibody (CAB15735) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using VDAC1 / Porin antibody (CAB15735) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH/3T3 cells using VDAC1 / Porin antibody (CAB15735) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-VDAC1 / Porin Antibody (CAB0810)

Anti-VDAC1 / Porin Antibody (CAB13638)

Anti-VDAC1 Antibody (CAB19707)

VDAC1 Antibody (PACO02076)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)