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Anti-BCHE Antibody (CAB1460)

SKU:
CAB1460
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 5 Anti-BCHE Antibody CAB1460
  • Cell Biology Antibodies 5 Anti-BCHE Antibody CAB1460
  • Cell Biology Antibodies 5 Anti-BCHE Antibody CAB1460
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-BCHE Antibody
Antibody SKU:CAB1460
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 29-270 of human BCHE (NP_000046.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, SW620
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 29-270 of human BCHE (NP_000046.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EDDI IIAT KNGK VRGM NLTV FGGT VTAF LGIP YAQP PLGR LRFK KPQS LTKW SDIW NATK YANS CCQN IDQS FPGF HGSE MWNP NTDL SEDC LYLN VWIP APKP KNAT VLIW IYGG GFQT GTSS LHVY DGKF LARV ERVI VVSM NYRV GALG FLAL PGNP EAPG NMGL FDQQ LALQ WVQK NIAA FGGN PKSV TLFG ESAG AASV SLHL LSPG SHSL FTRA ILQS GSFN APWA VTSL YEAR NR
Gene ID:590
Uniprot:P06276
Cellular Location:Secreted
Calculated MW:68kDa
Observed MW:68kDa
Synonyms:BCHE, CHE1, CHE2, E1
Background: Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage.
UniProt Protein Function:BCHE: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency). BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. Belongs to the type-B carboxylesterase/lipase family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Hydrolase; Nuclear envelope; Secreted; EC 3.1.1.8

Chromosomal Location of Human Ortholog: 3q26.1-q26.2

Cellular Component: membrane; nuclear envelope lumen; endoplasmic reticulum lumen; extracellular region

Molecular Function:identical protein binding; choline binding; enzyme binding; cholinesterase activity; beta-amyloid binding; acetylcholinesterase activity; catalytic activity

Biological Process: response to drug; response to alkaloid; negative regulation of cell proliferation; choline metabolic process; cocaine metabolic process; cellular protein metabolic process; response to folic acid; response to glucocorticoid stimulus; neuroblast differentiation; learning; negative regulation of synaptic transmission

NCBI Summary:This gene encodes a cholinesterase enzyme and member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine. [provided by RefSeq, Jul 2016]
UniProt Code:P06276
NCBI GenInfo Identifier:116353
NCBI Gene ID:590
NCBI Accession:P06276.1
UniProt Related Accession:P06276
Molecular Weight:
NCBI Full Name:Cholinesterase
NCBI Synonym Full Names:butyrylcholinesterase
NCBI Official Symbol:BCHE  
NCBI Official Synonym Symbols:E1; CHE1; CHE2; BCHED  
NCBI Protein Information:cholinesterase
UniProt Protein Name:Cholinesterase
UniProt Synonym Protein Names:Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II; Pseudocholinesterase
Protein Family:Cholinesterase
UniProt Gene Name:BCHE  
UniProt Entry Name:CHLE_HUMAN
Anti-BCHE Antibody (CAB1460)Western blot analysis of extracts of various cell lines, using BCHE antibody (CAB1460) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-BCHE Antibody (CAB1460)Immunohistochemistry of paraffin-embedded rat kidney using BCHE antibody (CAB1460) at dilution of 1:100 (40x lens).
Anti-BCHE Antibody (CAB1460)Immunofluorescence analysis of U2OS cells using BCHE antibody (CAB1460). Blue: DAPI for nuclear staining.

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