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Anti-C5AR1 Antibody (CAB1900)

SKU:
CAB1900
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 7 Anti-C5AR1 Antibody CAB1900
  • Cell Biology Antibodies 7 Anti-C5AR1 Antibody CAB1900
$167 - $503
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Description

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Antibody Name:Anti-C5AR1 Antibody
Antibody SKU:CAB1900
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 250-350 of human C5AR1 (NP_001727.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, PC-3, U-87MG, Mouse liver, Rat lung, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 250-350 of human C5AR1 (NP_001727.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SFFI FWLP YQVT GIMM SFLE PSSP TFLL LNKL DSLC VSFA YINC CINP IIYV VAGQ GFQG RLRK SLPS LLRN VLTE ESVV RESK SFTR STVD TMAQ KTQA V
Gene ID:728
Uniprot:P21730
Cellular Location:Cell membrane, Cytoplasmic vesicle, Multi-pass membrane protein
Calculated MW:39kDa
Observed MW:45KDa
Synonyms:C5AR1, C5A, C5AR, C5R1, CD88
Background:
UniProt Protein Function:C5aR: a family 1 G-protein coupled receptor that stimulates the GTPase activity of Gi2. Receptor for the chemotactic and inflammatory complement factor C5a. Stimulates chemotaxis, granule enzyme release and superoxide anion production. Phosphorylated in response to C5a or after stimulation of cells with phorbol esters. Expressed on monocytes, granulocytes, dendritic cells, astrocytes and microglia.
UniProt Protein Details:

Protein type:Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: cell surface; basolateral plasma membrane; integral to plasma membrane; apical part of cell; cytoplasmic membrane-bound vesicle; plasma membrane

Molecular Function:complement component C5a binding; complement component C5a receptor activity; C5a anaphylatoxin receptor activity

Biological Process: neutrophil chemotaxis; sensory perception of chemical stimulus; organ regeneration; apoptosis; activation of MAPK activity; response to lipopolysaccharide; cell proliferation in hindbrain; signal transduction; chemotaxis; mRNA transcription from RNA polymerase II promoter; defense response to Gram-positive bacterium; elevation of cytosolic calcium ion concentration; phospholipase C activation; response to peptidoglycan; cellular defense response; immune response; negative regulation of neuron apoptosis; inflammatory response; positive regulation of epithelial cell proliferation

UniProt Code:P21730
NCBI GenInfo Identifier:311033355
NCBI Gene ID:728
NCBI Accession:P21730.2
UniProt Related Accession:P21730
Molecular Weight:39,336 Da
NCBI Full Name:C5a anaphylatoxin chemotactic receptor 1
NCBI Synonym Full Names:complement component 5a receptor 1
NCBI Official Symbol:C5AR1  
NCBI Official Synonym Symbols:C5A; C5AR; C5R1; CD88  
NCBI Protein Information:C5a anaphylatoxin chemotactic receptor 1; C5a-R; C5a ligand; C5a anaphylatoxin receptor; complement component 5 receptor 1
UniProt Protein Name:C5a anaphylatoxin chemotactic receptor 1
UniProt Synonym Protein Names:C5a anaphylatoxin chemotactic receptor; C5a-R; C5aR; CD_antigen: CD88
Protein Family:C5a anaphylatoxin chemotactic receptor
UniProt Gene Name:C5AR1  
UniProt Entry Name:C5AR1_HUMAN
Anti-C5AR1 Antibody (CAB1900)Western blot analysis of extracts of various cell lines, using C5AR1 antibody (CAB1900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-C5AR1 Antibody (CAB1900)Western blot analysis of extracts of various cell lines, using C5AR1 antibody (CAB1900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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