Anti-CPE Antibody (CAB5458)

Antibody Name:	Anti-CPE Antibody
Antibody SKU:	CAB5458
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 257-476 of human CPE (NP_001864.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	A-549, MCF7, Mouse brain, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 257-476 of human CPE (NP_001864.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PYDE TRSG SAHE YSSS PDDA IFQS LARA YSSF NPAM SDPN RPPC RKND DDSS FVDG TTNG GAWY SVPG GMQD FNYL SSNC FEIT VELS CEKF PPEE TLKT YWED NKNS LISY LEQI HRGV KGFV RDLQ GNPI ANAT ISVE GIDH DVTS AKDG DYWR LLIP GNYK LTAS APGY LAIT KKVA VPYS PAAG VDFE LESF SERK EEEK EELM EWWK MMSE TLNF
Gene ID:	1363
Uniprot:	P16870
Cellular Location:	Cytoplasmic vesicle, Nucleus, Peripheral membrane protein, Secreted, secretory vesicle membrane
Calculated MW:	49kDa/53kDa
Observed MW:	53-55kDa

Synonyms:

CPE, CPH

Background:

This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes.

UniProt Protein Function:	CPE: Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protease; EC 3.4.17.10; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 4q32.3 Cellular Component: cell soma; extracellular space; Golgi apparatus; plasma membrane; secretory granule; secretory granule membrane Molecular Function:carboxypeptidase activity; cell adhesion molecule binding; metallocarboxypeptidase activity; neurexin binding; serine carboxypeptidase activity Biological Process: insulin processing; metabolic process; Wnt receptor signaling pathway
NCBI Summary:	This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes. [provided by RefSeq, Nov 2015]
UniProt Code:	P16870
NCBI GenInfo Identifier:	115892
NCBI Gene ID:	1363
NCBI Accession:	P16870.1
UniProt Secondary Accession:	P16870,Q9UIU9, A8K4N1, B3KR42, B4DFN4, D3DP33,
UniProt Related Accession:	P16870
Molecular Weight:	49,886 Da
NCBI Full Name:	Carboxypeptidase E
NCBI Synonym Full Names:	carboxypeptidase E
NCBI Official Symbol:	CPE
NCBI Official Synonym Symbols:	CPH
NCBI Protein Information:	carboxypeptidase E
UniProt Protein Name:	Carboxypeptidase E
UniProt Synonym Protein Names:	Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase
Protein Family:	Carboxypeptidase
UniProt Gene Name:	CPE
UniProt Entry Name:	CBPE_HUMAN

	Western blot analysis of extracts of various cell lines, using CPE antibody (CAB5458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using CPE antibody (CAB5458). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
CPE Antibody (PACO01881)	Human Carboxypeptidase E / CPE ELISA Kit
CPE Antibody (PACO06372)	Human CPE (Carboxypeptidase E) CLIA Kit (HUES00250)
CPE Antibody (PACO08563)	Human CPE (Carboxypeptidase E) ELISA Kit (HUES01569)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)