GAPDH Antibody (PACO49618)
- SKU:
- PACO49618
- Product type:
- Antibody
- Reactivity:
- Cricetulus griseus
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | GAPDH Antibody (PACO49618) |
| Antibody SKU: | PACO49618 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000 |
| Species Reactivity: | Cricetulus griseus |
| Immunogen: | Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase protein (2-333AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in Recombinant protein. All lanes: GAPDH antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. predicted band size: 36 kDa. observed band size: 36 kDa.. |
| Background: | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (γ interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-γ treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (By similarity). |
| Synonyms: | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99), GAPDH, GAPD |
| UniProt Protein Function: | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (). |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P17244 |
| NCBI GenInfo Identifier: | 349501082 |
| NCBI Gene ID: | 100736557 |
| NCBI Accession: | NP_001231783.1 |
| UniProt Secondary Accession: | P17244 |
| UniProt Related Accession: | P17244 |
| Molecular Weight: | 35,748 Da |
| NCBI Full Name: | glyceraldehyde-3-phosphate dehydrogenase |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | Gapdh |
| NCBI Official Synonym Symbols: | GAPD |
| NCBI Protein Information: | glyceraldehyde-3-phosphate dehydrogenase |
| UniProt Protein Name: | Glyceraldehyde-3-phosphate dehydrogenase |
| UniProt Synonym Protein Names: | Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-) |
| Protein Family: | |
| UniProt Gene Name: | GAPDH |
| UniProt Entry Name: | G3P_CRIGR |
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