Anti-HARS Antibody (PACO43522)

Antibody Name:	HARS Antibody (PACO43522)
Antibody SKU:	PACO43522
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IP
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000
Species Reactivity:	Human
Immunogen:	Recombinant Human Histidine--tRNA ligase, cytoplasmic protein (1-180AA)

Form:	Liquid
Storage Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method:	Antigen Affinity Purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western blot. All lanes: HARS antibody at 6µg/ml. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 58, 53, 51, 55 kDa. Observed band size: 58 kDa.
	Immunohistochemistry of paraffin-embedded human liver cancer using PACO43522 at dilution of 1:100.
	Immunohistochemistry of paraffin-embedded human colon tissue using PACO43522 at dilution of 1:100.

Background:

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acid,. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene.

Synonyms:

Histidine--tRNA ligase, cytoplasmic (EC 6.1.1.21) (Histidyl-tRNA synthetase) (HisRS), HARS, HRS

UniProt Protein Function:	HARS: Defects in HARS are a cause of Usher syndrome type 3B (USH3B). USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life. Belongs to the class-II aminoacyl-tRNA synthetase family.
UniProt Protein Details:	Protein type:EC 6.1.1.21; Translation; Ligase Chromosomal Location of Human Ortholog: 5q31.3 Cellular Component: cytoplasm; cytosol; mitochondrion Molecular Function:histidine-tRNA ligase activity Biological Process: histidyl-tRNA aminoacylation; mitochondrial translation; tRNA aminoacylation for protein translation Disease: Charcot-marie-tooth Disease, Axonal, Type 2w; Usher Syndrome, Type Iiib
NCBI Summary:	Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:	P12081
NCBI GenInfo Identifier:	135123
NCBI Gene ID:	3035
NCBI Accession:	P12081.2
UniProt Secondary Accession:	P12081,B4DHQ1, B4DY73, D6REN6, J3KNE5,
UniProt Related Accession:	P12081
Molecular Weight:	54,847 Da
NCBI Full Name:	Histidine--tRNA ligase, cytoplasmic
NCBI Synonym Full Names:	histidyl-tRNA synthetase
NCBI Official Symbol:	HARS
NCBI Official Synonym Symbols:	HRS; CMT2W; USH3B
NCBI Protein Information:	histidine--tRNA ligase, cytoplasmic
UniProt Protein Name:	Histidine--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names:	Histidyl-tRNA synthetase; HisRS
Protein Family:	Histidine--tRNA ligase
UniProt Gene Name:	HARS
UniProt Entry Name:	SYHC_HUMAN

Antibodies	ELISA Kits
Anti-HARS Antibody (CAB7899)	Human HARS / Histidyl-tRNA synthetase, cytoplasmic ELISA Kit
HARS Antibody (PACO00907)	Human Histidyl-tRNA synthetase, cytoplasmic (HARS) ELISA Kit
HARS Antibody (PACO09671)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)