Iron Assay Kit (BA0040)

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SKU:
BA0040
€572

Description

ELISA Kit Technical ManualMSDS

Iron Assay Kit - Description

Assay Genie's iron assay kit is designed to measure total iron directly in serum without any pretreatment. The improved method utilizes a chromogen that forms a blue colored complex specifically with Fe2+. Fe3+ in the sample is reduced to Fe2+, thus allowing the assay for total iron concentration. The intensity of the color, measured at 590nm, is directly proportional to the iron concentration in the sample.

Applications

For quantitative determination of iron ions Fe3+ and/or Fe2+ and evaluation of drug effects on iron metabolism.

Iron Assay Kit - Key Features

  • Sensitive and accurate. Linear detection range 27 ug/dL (4.8 ) to 1,000 ug/dL (179 ) iron in 96-well plate assay.
  • Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 40 min. Can be readily automated as a high-throughput assay for thousands of samples per day.
  • Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay.
  • Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples.

Iron Assay Kit - Data Sheet

Kit IncludesReagent A: 50 mL Reagent B: 4 mL Reagent C: 4 mL Iron Standard: 1 mL 10 mg/dL Fe2+
Kit RequiresPipeting devices and accessories.
Method of DetectionOD590nm
Detection Limit27 ug/dL (4.8 )
SamplesBiological (e.g. serum) and environmental samples
SpeciesAll
Protocol Length30 min
Size250 tests
StorageStore Reagent A at room temperature and other reagents at 4 °C.
Shelf Life12 months

More Details

Iron level in blood is a reliable diagnostic indicator of various disease states. Increased levels of iron concentration in blood are associated with blood loss, increased destruction of red blood cells (e.g. hemorrhage) or decreased blood cell survival, acute hepatitis, certain sideroachrestic anemias, ingestion of iron-rich diets, defects in iron storage (e.g. pernicious anemia). Decreased levels of blood iron may result from insufficient iron ingestion from diets, chronic blood loss pathologies, or increased demand on iron storage as during normal pregnancy. Simple, direct and automation-ready procedures for measuring iron concentrations find wide applications in research, drug discovery and environmental monitoring.
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