Anti-JMJD6 Antibody (CAB5840)

Antibody Name:	Anti-JMJD6 Antibody [KO Validated]
Antibody SKU:	CAB5840
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human JMJD6 (NP_055982.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human JMJD6 (NP_055982.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNHK SKKR IREA KRSA RPEL KDSL DWTR HNYY ESFS LSPA AVAD NVER ADAL QLSV EEFV ERYE RPYK PVVL LNAQ EGWS AQEK WTLE RLKR KYRN QKFK CGED NDGY SVKM KMKY YIEY MEST RDDS PLYI FDSS YGEH PKRR KLLE DYKV PKFF TDDL FQYA GEKR RPPY RWFV MGPP RSGT GIHI DPLG TSAW NALV QGHK RWCL FPTS TPRE LIKV TRDE GGNQ QDEA ITWF NVIY PRTQ LPTW PPEF KPLE ILQK PGET VFVP GGWW HVVL NLDT TIAI TQNF ASST NFPV VWHK TVRG RPKL SRKW YRIL KQEH
Gene ID:	23210
Uniprot:	Q6NYC1
Cellular Location:	Nucleus, nucleolus, nucleoplasm
Calculated MW:	43kDa/46kDa/47kDa
Observed MW:	46KDa/49KDa

Synonyms:

JMJD6, PSR, PTDSR, PTDSR1

Background:

This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. This protein was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have indicated that it does not directly function in the clearance of apoptotic cells, and questioned whether it is a true phosphatidylserine receptor. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	JMJD6: Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses. Belongs to the JMJD6 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Receptor, misc.; Cell development/differentiation; EC 1.14.11.-; Apoptosis; Oxidoreductase; Nucleolus Chromosomal Location of Human Ortholog: 17q25 Cellular Component: nucleoplasm; plasma membrane; nucleolus; nucleus Molecular Function:identical protein binding; protein binding; histone demethylase activity (H4-R3 specific); single-stranded RNA binding; RNA binding; iron ion binding; histone demethylase activity (H3-R2 specific); receptor activity Biological Process: erythrocyte development; transcription, DNA-dependent; heart development; RNA splicing; T cell differentiation in the thymus; recognition of apoptotic cell; peptidyl-lysine hydroxylation to 5-hydroxy-L-lysine; macrophage activation; regulation of nuclear mRNA splicing, via spliceosome; cell surface receptor linked signal transduction; regulation of transcription, DNA-dependent; retina development in camera-type eye; sprouting angiogenesis; mRNA processing; kidney development; lung development
NCBI Summary:	This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. This protein was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have indicated that it does not directly function in the clearance of apoptotic cells, and questioned whether it is a true phosphatidylserine receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q6NYC1
NCBI GenInfo Identifier:	67461014
NCBI Gene ID:	23210
NCBI Accession:	Q6NYC1.1
UniProt Secondary Accession:	Q6NYC1,Q86VY0, Q8IUM5, Q9Y4E2, B3KMN8, B4DGX1,
UniProt Related Accession:	Q6NYC1
Molecular Weight:	47,626 Da
NCBI Full Name:	Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
NCBI Synonym Full Names:	jumonji domain containing 6
NCBI Official Symbol:	JMJD6
NCBI Official Synonym Symbols:	PSR; PTDSR; PTDSR1
NCBI Protein Information:	bifunctional arginine demethylase and lysyl-hydroxylase JMJD6; phosphatidylserine receptor; jmjC domain-containing protein 6; histone arginine demethylase JMJD6; peptide-lysine 5-dioxygenase JMJD6; jumonji domain-containing protein 6
UniProt Protein Name:	Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
UniProt Synonym Protein Names:	Histone arginine demethylase JMJD6; JmjC domain-containing protein 6; Jumonji domain-containing protein 6; Lysyl-hydroxylase JMJD6; Peptide-lysine 5-dioxygenase JMJD6; Phosphatidylserine receptor; Protein PTDSR
Protein Family:	Bifunctional arginine demethylase and lysyl-hydroxylase
UniProt Gene Name:	JMJD6
UniProt Entry Name:	JMJD6_HUMAN

	Western blot analysis of extracts of 293T cells, using JMJD6 antibody (CAB5840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded rat brain using JMJD6 Rabbit pAb (CAB5840) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung using JMJD6 Rabbit pAb (CAB5840) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spinal cord using JMJD6 Rabbit pAb (CAB5840) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
JMJD6 Antibody (PACO10028)	Human Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 (JMJD6) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)