Anti-MC1R Antibody (CAB3009)

Antibody Name:	Anti-MC1R Antibody
Antibody SKU:	CAB3009
Antibody Size:	20µL, 50µL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant protein of human MC1R

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	M21, MCF7, SW620, 22Rv1, Raji, Mouse testis

Immunogen:	Recombinant protein of human MC1R
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4157
Uniprot:	Q01726
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	34kDa
Observed MW:	37kDa

Synonyms:

MC1R, CMM5, MSH-R, SHEP2

Background:

This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation.

UniProt Protein Function:	Function: Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
UniProt Protein Details:	Subunit structure: Interacts with MGRN1, but does not undergo MGRN1-mediated ubiquitination; this interaction competes with GNAS-binding and thus inhibits agonist-induced cAMP production. Ref.14 Subcellular location: Cell membrane; Multi-pass membrane protein. Tissue specificity: Melanocytes and corticoadrenal tissue. Polymorphism: Genetic variants in MC1R define the skin/hair/eye pigmentation variation locus 2 (SHEP2) [ MIM:266300]. Hair, eye and skin pigmentation are among the most visible examples of human phenotypic variation, with a broad normal range that is subject to substantial geographic stratification. In the case of skin, individuals tend to have lighter pigmentation with increasing distance from the equator, with type I skin being the most lightly pigmented and type IV the most dark pigmented. By contrast, the majority of variation in human eye and hair color is found among individuals of European ancestry, with most other human populations fixed for brown eyes and black hair. Partial loss-of-function mutations are associated with fair skin, poor tanning and increased skin cancer risk.MC1R variants associated with red hair and fair skin, determine female-specific increased analgesia from kappa-opioid receptor agonist [ MIM:613098]. Involvement in Disease: Melanoma, cutaneous malignant 5 (CMM5) [MIM:613099]: A malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.24 Ref.27 Sequence similarities: Belongs to the G-protein coupled receptor 1 family.
NCBI Summary:	This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation. [provided by RefSeq, Jul 2008]
UniProt Code:	Q01726
NCBI GenInfo Identifier:	12644376
NCBI Gene ID:	4157
NCBI Accession:	Q01726.2
UniProt Secondary Accession:	Q01726,Q66K38, Q8WWX6, Q8WWX7, Q96I33, Q96RU4, Q9UBF7 Q9UN58, Q9UN59, Q9UN60, Q9UN61, Q9UN62,
UniProt Related Accession:	Q01726
Molecular Weight:	34,706 Da
NCBI Full Name:	Melanocyte-stimulating hormone receptor
NCBI Synonym Full Names:	melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor)
NCBI Official Symbol:	MC1R
NCBI Official Synonym Symbols:	CMM5; MSH-R; SHEP2
NCBI Protein Information:	melanocyte-stimulating hormone receptor; MC1-R; melanotropin receptor
UniProt Protein Name:	Melanocyte-stimulating hormone receptor
UniProt Synonym Protein Names:	Melanocortin receptor 1
Protein Family:	Melanocyte-stimulating hormone receptor
UniProt Gene Name:	MC1R
UniProt Entry Name:	MSHR_HUMAN

Western blot analysis of extracts of various cell lines, using MC1R antibody (CAB3009) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

Anti-MC1R Antibody (CAB13152)

Anti-MC1R Antibody (CAB13512)

Anti-MC1 Receptor[KO Validated] Antibody (CAB11594)

MC1R Antibody (PACO02893)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)