Assay Genie’s Fluorometric L-lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at Ex/Em = 530/585 nm, is proportional to the lactate concentration in the sample.
For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.
- Sensitive and accurate. Detection limit of 1 μM and linearity up to 50 μM L-lactate in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
|Kit Includes||Assay Buffer: 10 mL Enzyme A: 120 mL NAD Solution: 1 mL Enzyme B: 120 mL Probe: 750 mL Standard: 1 mL|
|Kit Requires||Pipetting (multi-channel) devices. Black, flat bottom 96-well plates and fluorescent plate reader capable of reading at λex/em = 530/585 nm.|
|Method of Detection||FL530/585 nm|
|Detection Limit||1 μM|
|Samples||Serum, plasma, cell culture media etc|
|Protocol Length||60 min|
|Storage||Store all reagents at -20°C|
|Shelf Life||6 months|
LACTATE is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.