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Anti-F5 Antibody (CAB1292)

SKU:
CAB1292
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular
$167 - $503
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Description

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Antibody Name:Anti-F5 Antibody
Antibody SKU:CAB1292
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 300-510 of human F5 (NP_000121.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 300-510 of human F5 (NP_000121.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VGPE GKWI ISSL TPKH LQAG MQAY IDIK NCPK KTRN LKKI TREQ RRHM KRWE YFIA AEEV IWDY APVI PANM DKKY RSQH LDNF SNQI GKHY KKVM YTQY EDES FTKH TVNP NMKE DGIL GPII RAQV RDTL KIVF KNMA SRPY SIYP HGVT FSPY EDEV NSSF TSGR NNTM IRAV QPGE TYTY KWNI LEFD EPTE NDAQ CLTR PYYS DVD
Gene ID:2153
Uniprot:P12259
Cellular Location:Secreted
Calculated MW:251kDa
Observed MW:290kDa
Synonyms:F5, FVL, PCCF, RPRGL1, THPH2
Background:This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance.
UniProt Protein Function:factor V: Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. Defects in F5 are the cause of factor V deficiency (FA5D); also known as Owren parahemophilia. It is an hemorrhagic diastesis. Defects in F5 are the cause of thrombophilia due to activated protein C resistance (THPH2). THPH2 is a hemostatic disorder due to defective degradation of factor Va by activated protein C. It is characterized by a poor anticoagulant response to activated protein C resulting in tendency to thrombosis. Defects in F5 are a cause of susceptibility to Budd- Chiari syndrome (BDCHS). A syndrome caused by obstruction of hepatic venous outflow involving either the hepatic veins or the terminal segment of the inferior vena cava. Obstructions are generally caused by thrombosis and lead to hepatic congestion and ischemic necrosis. Clinical manifestations observed in the majority of patients include hepatomegaly, right upper quadrant pain and abdominal ascites. Budd-Chiari syndrome is associated with a combination of disease states including primary myeloproliferative syndromes and thrombophilia due to factor V Leiden, protein C deficiency and antithrombin III deficiency. Budd-Chiari syndrome is a rare but typical complication in patients with polycythemia vera. Defects in F5 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F5 are associated with susceptibility to pregnancy loss, recurrent, type 1 (RPRGL1). RPRGL1 is a common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the multicopper oxidase family.
UniProt Protein Details:

Protein type:Secreted; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: endoplasmic reticulum lumen; ER to Golgi transport vesicle; ER-Golgi intermediate compartment membrane; extracellular region; extracellular space; Golgi membrane; membrane; plasma membrane

Molecular Function:copper ion binding; protein binding

Biological Process: blood circulation; blood coagulation; cellular protein metabolic process; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; platelet activation; platelet degranulation; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine

Disease: Budd-chiari Syndrome; Factor V Deficiency; Pregnancy Loss, Recurrent, Susceptibility To, 1; Stroke, Ischemic; Thrombophilia Due To Activated Protein C Resistance

NCBI Summary:This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance. [provided by RefSeq, Oct 2008]
UniProt Code:P12259
NCBI GenInfo Identifier:105990535
NCBI Gene ID:2153
NCBI Accession:NP_000121.2
UniProt Secondary Accession:P12259,Q14285, Q2EHR5, Q5R346, Q5R347, Q6UPU6, Q8WWQ6 A8K6E8,
UniProt Related Accession:P12259
Molecular Weight:30.4 kDa
NCBI Full Name:coagulation factor V preproprotein
NCBI Synonym Full Names:coagulation factor V
NCBI Official Symbol:F5  
NCBI Official Synonym Symbols:FVL; PCCF; THPH2; RPRGL1  
NCBI Protein Information:coagulation factor V
UniProt Protein Name:Coagulation factor V
UniProt Synonym Protein Names:Activated protein C cofactor; Proaccelerin, labile factor
Protein Family:Coagulation factor
UniProt Gene Name:F5  
UniProt Entry Name:FA5_HUMAN
Anti-F5 Antibody (CAB1292)Western blot analysis of extracts of HepG2 cells, using F5 antibody (CAB1292) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 240s.

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