Anti-HCST Antibody (CAB17887)

Antibody Name:	Anti-HCST Antibody
Antibody SKU:	CAB17887
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-93 of human HCST (NP_055081.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-93 of human HCST (NP_055081.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MIHL GHIL FLLL LPVA AAQT TPGE RSSL PAFY PGTS GSCS GCGS LSLP LLAG LVAA DAVA SLLI VGAV FLCA RPRR SPAQ EDGK VYIN MPGR G
Gene ID:	10870
Uniprot:	Q9UBK5
Cellular Location:
Calculated MW:	9kDa
Observed MW:	Refer to figures

Synonyms:

DAP10, KAP10, PIK3AP, HCST

Background:

This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms.

UniProt Protein Function:	Transmembrane adapter protein which associates with KLRK1 to form an activation receptor KLRK1-HCST in lymphoid and myeloid cells; this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs); these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with KLRK1-HCST triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full KLRK1-HCST-mediated activation and ultimate killing of target cells. In NK cells, KLRK1-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. KLRK1-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells; indeed, melanoma cells that do not express KLRK1 ligands escape from immune surveillance mediated by NK cells.
NCBI Summary:	This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UBK5
NCBI GenInfo Identifier:	74734930
NCBI Gene ID:	10870
NCBI Accession:	Q9UBK5.1
UniProt Related Accession:	Q9UBK5
Molecular Weight:	9kDa
NCBI Full Name:	Hematopoietic cell signal transducer
NCBI Synonym Full Names:	hematopoietic cell signal transducer
NCBI Official Symbol:	HCST
NCBI Official Synonym Symbols:	DAP10; KAP10; PIK3AP
NCBI Protein Information:	hematopoietic cell signal transducer
UniProt Protein Name:	Hematopoietic cell signal transducer
UniProt Synonym Protein Names:	DNAX-activation protein 10; Membrane protein DAP10; Transmembrane adapter protein KAP10
Protein Family:	Hematopoietic cell signal transducer
UniProt Gene Name:	HCST

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)