Anti-INPP5B Antibody (CAB3927)

Antibody Name:	Anti-INPP5B Antibody
Antibody SKU:	CAB3927
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human INPP5B (NP_005531.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Raji, SGC7901, HepG2, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human INPP5B (NP_005531.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDQS VAIQ ETLA EGEY CVIA VQGV LCEG DSRQ SRLL GLVR YRLE HGGQ EHAL FLYT HRRM AITG DDVS LDQI VPVS RDFT LEEV SPDG ELYI LGSD VTVQ LDTA ELSL VFQL PFGS QTRM FLHE VARA CPGF DSAT RDPE FLWL SRYR CAEL ELEM PTPR GCNS ALVT WPGY ATIG GGGS NFDG LRPN GKGV PMDQ SSRG QDKP ESLQ PRQN KSKS EITD MVRS STIT VSDK AHIL SMQK FGLR DTIV KSHL LQKE EDYT
Gene ID:	3633
Uniprot:	P32019
Cellular Location:	Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Early endosome membrane, Endoplasmic reticulum-Golgi intermediate compartment, Golgi apparatus, Membrane, Peripheral membrane protein, cytosol, phagosome membrane
Calculated MW:	85kDa/93kDa/103kDa/112kDa
Observed MW:	113kDa

Synonyms:

INPP5B, 5PTase

Background:

This gene encodes a member of a family of inositol polyphosphate-5-phosphatases. These enzymes function in the regulation of calcium signaling by inactivating inositol phosphates. The encoded protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Alternatively spliced transcript variants of this gene have been described.

UniProt Protein Function:	INPP5B: Hydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events. Belongs to the inositol 1,4,5-trisphosphate 5- phosphatase type II family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - inositol phosphate; EC 3.1.3.36; Phosphatase (non-protein); Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1p34 Cellular Component: Golgi apparatus; phagocytic vesicle membrane; membrane; early endosome membrane; cytoplasm; integral to membrane; plasma membrane; ER-Golgi intermediate compartment; cytosol Molecular Function:protein binding; metal ion binding; phosphoinositide 5-phosphatase activity Biological Process: sperm motility; regulation of small GTPase mediated signal transduction; inositol phosphate metabolic process; in utero embryonic development; small GTPase mediated signal transduction; phosphoinositide dephosphorylation; spermatogenesis
NCBI Summary:	This gene encodes a member of a family of inositol polyphosphate-5-phosphatases. These enzymes function in the regulation of calcium signaling by inactivating inositol phosphates. The encoded protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jul 2014]
UniProt Code:	P32019
NCBI GenInfo Identifier:	37590185
NCBI Gene ID:	3633
NCBI Accession:	BC058932
UniProt Secondary Accession:	P32019,Q5VSG9, Q5VSH0, Q5VSH1, Q658Q5, Q6P6D4, Q6PD53 Q86YE1, C9J6U5,
UniProt Related Accession:	P32019
Molecular Weight:	Calculated MW: 113 kDaObserved MW: 75 kDa
NCBI Full Name:	INPP5B protein
NCBI Synonym Full Names:	inositol polyphosphate-5-phosphatase, 75kDa
NCBI Official Symbol:	INPP5B
NCBI Official Synonym Symbols:	5PTase
NCBI Protein Information:	type II inositol 1,4,5-trisphosphate 5-phosphatase; phosphoinositide 5-phosphatase
UniProt Protein Name:	Type II inositol 1,4,5-trisphosphate 5-phosphatase
UniProt Synonym Protein Names:	75 kDa inositol polyphosphate-5-phosphatase; Phosphoinositide 5-phosphatase; 5PTase
Protein Family:	Type II inositol 1,4,5-trisphosphate 5-phosphatase
UniProt Gene Name:	INPP5B
UniProt Entry Name:	I5P2_HUMAN

	Western blot analysis of extracts of various cell lines, using INPP5B antibody (CAB3927) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of C6 cells using INPP5B antibody (CAB3927) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using INPP5B antibody (CAB3927) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

INPP5B Antibody (PACO09962)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)