Anti-LGMN Antibody (PACO57836)

Antibody Name:	LGMN Antibody (PACO57836)
Antibody SKU:	PACO57836
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:500-1:1000
Species Reactivity:	Human
Immunogen:	Recombinant Human Legumain protein (199-312AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO57836 diluted at 1:600 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of PACO57836 diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acid, c conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity). May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

Synonyms:

Legumain (EC 3.4.22.34) (Asparaginyl endopeptidase) (Protease, cysteine 1), LGMN, PRSC1

UniProt Protein Function:	LGMN: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Belongs to the peptidase C13 family.
UniProt Protein Details:	Protein type:EC 3.4.22.34; Protease Chromosomal Location of Human Ortholog: 14q32.12 Cellular Component: lysosomal lumen; lysosome Molecular Function:cysteine-type endopeptidase activity; peptidase activity Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; negative regulation of neuron apoptosis; proteolysis; proteolysis involved in cellular protein catabolic process; receptor catabolic process; renal system process; toll-like receptor signaling pathway; vacuolar protein processing; vitamin D metabolic process
NCBI Summary:	This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99538
NCBI GenInfo Identifier:	2842759
NCBI Gene ID:	5641
NCBI Accession:	Q99538.1
UniProt Secondary Accession:	Q99538,O00123, Q86TV2, Q86TV3, Q9BTY1,
UniProt Related Accession:	Q99538
Molecular Weight:	Observed MW: 54kDaCalculated MW: 42kDa/49kDa
NCBI Full Name:	Legumain
NCBI Synonym Full Names:	legumain
NCBI Official Symbol:	LGMN
NCBI Official Synonym Symbols:	AEP; LGMN1; PRSC1
NCBI Protein Information:	legumain
UniProt Protein Name:	Legumain
UniProt Synonym Protein Names:	Asparaginyl endopeptidase; Protease, cysteine 1
UniProt Gene Name:	LGMN

Antibodies	ELISA Kits
Anti-LGMN Antibody (CAB10570)	Human LGMN / Legumain ELISA Kit
	Human Legumain (LGMN) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)