Human TFPI (Tissue Factor Pathway Inhibitor) CLIA Kit
The Human TFPI (Tissue Factor Pathway Inhibitor) CLIA Kit is specifically designed to accurately measure TFPI levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing dependable and consistent results for various research purposes.TFPI is a critical regulator of the blood coagulation pathway, inhibiting tissue factor-induced coagulation and promoting anticoagulant activity.
Dysregulation of TFPI has been implicated in various clotting disorders and cardiovascular diseases, emphasizing its importance as a biomarker for understanding these conditions and exploring therapeutic interventions.Overall, the Human TFPI CLIA Kit provides researchers with a valuable tool for studying the role of TFPI in health and disease, contributing to advancements in the field of hemostasis and thrombosis research.
Product Name:
Human TFPI (Tissue Factor Pathway Inhibitor) CLIA Kit
SKU:
HUES00142
Target:
Human TFPI (Tissue Factor Pathway Inhibitor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TFPI. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TFPI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TFPI, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TFPI. You can calculate the concentration of Human TFPI in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
94-106
97-111
Average (%)
92
101
105
1:4
Range (%)
98-112
89-102
101-113
Average (%)
104
95
107
1:8
Range (%)
90-101
100-114
91-104
Average (%)
95
108
96
1:16
Range (%)
94-106
99-112
102-117
Average (%)
100
105
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
95-109
103
Cell culture media (n=5)
97-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.02
278.52
872.74
100.06
252.51
820.7
Standard deviation
12.23
29.86
69.91
9.28
24.27
89.54
C V (%)
11.87
10.72
8.01
9.27
9.61
10.91
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TFPI concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TFPI in samples. No significant cross-reactivity or interference between Human TFPI and analogues was observed.
