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Molecular Biology · Gene SilencingWhat is RNAi? A Complete Guide to RNA InterferenceRNA interference (RNAi) is a conserved biological process in which small double-stranded RNA molecules silence gene expression by triggering the degradation of complementary messenger RNA. Also called post-transcriptional gene silencing, RNAi is both a natural regulatory pathway and one of the most powerful tools in modern functional genomics and drug development.Explore RNAi pathway ELISA kits →2006Nobel Prize awarded for RNAi discovery21–23 nttypical length of an siRNA guide3RNAi mediators: siRNA, miRNA, shRNA6+approved RNAi therapeutics to dateKey TakeawaysRNAi is a natural process where double-stranded RN …

Leukemia Inhibitory Factor (LIF) is a cytokine that plays a crucial role in various physiological processes within the human body. It has gained significant attention in the field of leukemia research due to its multifaceted functions and potential therapeutic applications. In this blog the molecular structure, biological sources, signaling pathways, and the role of LIF in leukemia will be discussed. Table of Contents Jump to a section: - LIF Expression - LIF Signaling - LIF in Leukemia - LIF Related Kits - LIF in Stem Cell Culture .nav__list { list-style: none; } .nav__list a{ font-family: Popp …

Transfection protocols play a crucial role in the realm of molecular biology and genetic research, enabling the introduction of foreign nucleic acids into living cells. This innovative technique allows scientists to manipulate gene expression, study protein function, and investigate cellular processes with remarkable precision. This article focusses on the fundamental principles underlying transfection protocols, exploring their significance and various approaches employed for achieving successful genetic delivery Table of Contents Jump to a section: - Cell Transfection - Applications - Mechanism - Transfection vs Tr …

What is VEGF? Vascular Endothelial Growth Factors (VEGFs) are a group of homodimeric polypeptides, and are master regulators of vascular development, maintenance and angiogenesis. VEGF is also a potent growth factor, and is produced by macrophages and CD4+ and CD8+ T cells. Members of the VEGF family include 5 structurally related proteins (VEGFA-D, and placental growth factor (PIGF), which are further individually subdivided based on alternative splicing. VEGF acts as a potent stimulator of angiogenesis, promoting the growth and survival of endothelial cells, and is essential for embryonic development and tissue repair in adults. The discovery of VEGF and it …

Delving into the intricate world of Mitotic Catastrophe, this blog unveils its critical role in cell division and cancer therapy, offering insights into its mechanisms and potential treatments. Key Takeaways: Mitotic Catastrophe: A response to abnormal mitosis, potentially leading to cell death. Oncosuppressive Role: Prevents proliferation of genetically unstable cells, guarding against cancer. Mitotic Failure and Slippage: Involves death without mitosis exit or progression to G1 phase. Cyclin B Proteolysis: Key in mitotic slippage, leading to Cdk1 inactivation. Prolonged Arrest: Influences cell fate, with a cutoff point for irreversible catastrophe. Targeting Strategies …

Cell Cycle · KinasesCyclins and Cyclin-Dependent Kinases: Regulation, Substrates and Mitotic ControlCDKs are inactive on their own. They require a cyclin partner, and because cyclin levels rise and fall on a schedule, the cell cycle advances in a fixed order. This guide covers which cyclin pairs with which CDK, how the CDK1-cyclin B complex drives mitotic entry through positive feedback, the substrate motif that made proteomic mapping possible, the Cdk1-Plk1-Aurora B hierarchy, and how cyclin destruction ends mitosis.Browse cell cycle antibodies →1982Hunt discovers cyclins in sea urchinsCDK1–CDK20The renamed kinase familyS/T-P-X-K/RThe CDK1 substrate motifAPC/C–Cdc20Destroys cyclin B at anap …

What is Immunometabolism? Immunometabolism is an exponentially growing, multi-disciplinary field of research aiming at deciphering the dynamic cellular and molecular mechanisms that interweave metabolic and immunological processes together. Although the term “immunometabolism” first appeared in the literature in 2011, the first studies investigating the connection between immune and metabolic disorders date back to the late 19th century. Our expanding understanding of how different immune cell functions correlate with particular metabolic configurations during homeostasis and inflammation has opened new therapeutic possibilities where the modulation of …

The immune system plays a critical role in defending the body against pathogens and maintaining tissue homeostasis. Within the intricate network of immune cells, CD11b, also known as integrin alpha M, emerges as a significant player in immunology and neuroscience. CD11b serves as a cell surface marker that aids in identifying and characterizing specific immune cell populations, particularly macrophages and microglia. Understanding the role of CD11b and its association with these cells provides valuable insights into various disease processes, immune responses, and neuroinflammatory disorders. In this blog post, we will look at the structure and function of CD11b, its significance …

Pyruvate dehydrogenase is a complex enzyme involved in the conversion of pyruvate, a product of glycolysis, into acetyl-CoA, a crucial molecule in energy production and intermediary metabolism. Table of Contents Jump to a section: - PDH Structure & Function - PDH Regulation - PDH & Cancer - PDH Related Kits - PDH & PDC .nav__list { list-style: none; } .nav__list a{ font-family: Poppins; font-size: 18px; color: #29aae1; } .nav__list li{ margin-bottom: 20px; } .nav__list a:hover{ color:#0e2f51; } { "@context": "http …

What is a Plasma Membrane? The plasma membrane, also known as the cell membrane, is a vital component of all cells. It is a thin, semi-permeable barrier that separates the cell's internal environment from the external surroundings. This crucial structure plays a fundamental role in maintaining the integrity and functionality of the cell. { "@context": "https://schema.org", "@type": "BlogPosting", "mainEntityOfPage": { "@type": "WebPage", "@id": "https://www.assaygenie.com/plasma-membrane-markers" }, "headline": "Plasma Membrane Markers", "description": "Explore the role of plasma membrane markers in cell biology and disease …

Since I am from Ireland I thought I would give as comprehensive view as possible for Research Universities in Ireland. By now you are probably considering looking for a PhD or PostDoc position in Ireland which is a great start. Known as the Land of the scholars,there are 10 international standard Research Universities in Ireland and colleges that focus on a range of life science, chemistry, physics and engineering research. { "@context": "https://schema.org", "@type": "BlogPosting", "mainEntityOfPage": { "@type": "WebPage", "@id": "https://www.assaygenie.com/blog/research-universities-in-ireland/" }, "headline": "Research Universities in Ireland", "image": "" …

Immunology · Complement SystemThe Alternative Complement PathwayThe alternative pathway is one of three routes that activate complement — an antibody-independent, always-on surveillance system driven by spontaneous C3 tick-over and a powerful amplification loop. This guide covers its activation, regulation, key proteins and clinical significance.Browse Complement ELISA Kits →C3SPONTANEOUS TICK-OVERB + DCONVERTASE ASSEMBLYLoopAMPLIFICATIONMACMEMBRANE ATTACK COMPLEXQuick answerThe alternative complement pathway is an antibody-independent arm of innate immunity. It is continuously primed by spontaneous hydrolysis of C3 (“tick-over”), which on foreign surfaces recruits Factor B and Factor …

Cancer Biology · Cell Signallingp53: Structure, Signalling, Regulation and Mutation in Cancerp53 is the most frequently mutated gene in human cancer, and the reason is structural: a single tetrameric transcription factor sits at the junction between DNA damage detection and the decision to arrest, repair, or die. This guide covers p53’s domain architecture, how MDM2 keeps it switched off, the target genes it activates, and what changes when it is mutated.Browse p53 pathway kits →393Amino acids per p53 monomer17p13.1Chromosomal location of TP53TetramerThe active oligomeric state~50%Of human cancers carry TP53 mutationsKey takeawaysp53 is a tetrameric transcription factor encoded by TP53 on th …

What Are Personalized Cancer Vaccines? Personalized cancer vaccines are a cutting-edge approach in cancer treatment that are designed to activate a patient's immune system against their specific tumor. Unlike traditional vaccines that target infectious agents, personalized cancer vaccines are tailored to each individual's unique tumor characteristics. These vaccines are customized to exploit the specific genetic mutations and alterations present in the patient's tumor cells. How Do Personalized Cancer Vaccines Work? Personalized cancer vaccines work by utilizing neoantigens, which are mutated proteins expressed on the surface o …

Microbiology · ProbioticsLactobacilli: Structure, Metabolism, Identification and Probiotic UseLactobacilli are lactic acid bacteria found in fermented foods and across the human gastrointestinal, oral and vaginal tracts. This guide covers their cell structure, the homofermentative and heterofermentative metabolism that defines them, the 2020 taxonomic reclassification that renamed most familiar species, how they are correctly identified in the laboratory, and what the probiotic evidence does and does not support.Browse lactate assays →25Genera after the 2020 Lactobacillus splitL and DLactate stereoisomers, species-dependentHomo / heteroThe two fermentative strategiesCatalase-negativeKey iden …

Introduction Microtubules are key components of the cytoskeleton and are composed of alpha and beta tubulin. These dimerise in a head to tail fashion to form 13 linear protofilaments. The protofilaments associate initially into sheets and subsequently into hollow tubes with a diameter of roughly 25 nm. These hollow tubes radiate from the microtubule-organising centre (MTOC) located at the centrosome in interphase cells. Microtubules are essential in intracellular trafficking of vesicles and mitochondria, cell polarisation and migration. Furthermore, microtubules are involved in the development and maintenance of cell shape. In mitosis, microtubules undergo dynamic instabili …

What is Hepatocyte Growth Factor? Hepatocyte growth factor (HGF) is a cytokine that is mainly produced by the liver. HGF is a pleiotrophic cytokine that regulates cell growth, motility, and morphogenesis. The majority of HGF is produced by the liver, but it is also expressed in other organs, such as the kidney, pancreas, and intestine. HGF binds to its cognate receptor, c-Met, which is present on the surface of many types of cells. However, it has no measurable protease activity. Binding of HGF to c-Met leads to activation of multiple signaling pathways that promote cell proliferation, survival, migration, and differentiation. Key Takeaways Hepatocyte Growth Factor …

Exploring sepsis: a critical imbalance in the immune response to infection. Key Takeaways: Sepsis is a severe, immune-driven response to infection causing systemic inflammation and potential organ damage. It progresses through stages: initial sepsis, severe sepsis, and septic shock, each with increasing severity. Complications include organ failure, septic shock, ARDS, blood clots, and long-term impairments. Immune responses in sepsis vary from hypo-inflammation (weak) to hyper-inflammation (excessive). Understanding these responses is key for effective sepsis treatment and management. What is Sepsis? Sepsis is a life-threatening condition that arises when the bod …

Cell Signalling · Receptor BiologyGPCRs (G Protein-Coupled Receptors): A Complete GuideG protein-coupled receptors are the largest family of cell-surface receptors in the human genome and the target of roughly a third of all approved drugs. This guide covers GPCR structure, ligands, signalling pathways, regulation and pharmacology.Browse Research Antibodies →~800HUMAN GPCRs~34%OF APPROVED DRUGS TARGET GPCRs7TRANSMEMBRANE DOMAINS4G-PROTEIN CLASSESQuick answerGPCRs are seven-transmembrane receptors that convert extracellular signals — hormones, neurotransmitters, light and more — into intracellular responses through coupled G proteins and second messengers such as cyclic AMP and calcium. …

Sepsis, a life-threatening condition, is characterized by a dysregulated immune response to an infection, leading to systemic inflammation and organ dysfunction. Biomarkers, as measurable indicators of biological processes, hold immense potential in the field of sepsis diagnosis, prognosis, and management. In this article, we explore the critical role that biomarkers play in sepsis, particularly their significance in early detection and diagnosis. We delve into the latest research and advancements in biomarker identification, their association with the immune response duri …

Exploring DNA's essence, this guide illuminates its structure, function, and purification techniques, essential in fields like medicine and biotechnology. Key Takeaways: DNA's Structure: Composed of nucleotides adenine, thymine, cytosine, guanine, forming a double helix. Charge Properties: DNA exhibits a negative charge due to phosphate groups. Central Dogma: DNA's role in protein synthesis and inheritance. Extraction Methods: Techniques for isolating DNA. Purification Techniques: Ensuring DNA's integrity for analysis. Genomic DNA: The complete genetic information in cells. DNA Assays: Tools for DNA analysis and research applications. { "@context": "https://schema. …

Introduction Transformation of bacteria with plasmids is not only of note for bacterial studies but also can be used for gene expression studies in mammalian cells. Most plasmids are of bacterial origin and contain both a bacterial origin of replication and an antibiotic resistance gene which can be used as a selectable marker. The process of transformation allows foreign DNA to be introduced into a cell. Genetic modifications can be made to various bacterial strains to make them more amenable to transformation. Such modifications will maintain the plasmid without rearranging the plasmid DNA. Certain treatments have been shown to increase the transformation efficiency of bacteria. …

What is Gut Inflammation? The gut, or gastrointestinal (GI) tract is a long tube that starts from the mouth and extends to the anus. It includes various organs such as the esophagus, stomach, small intestine, large intestine, and rectum, responsible for digestion, absorption of nutrients, and elimination of waste. The gut plays a vital role in our overall health. However, inflammation can occur within the gut, leading to various inflammatory diseases. Gut inflammation is an immune response triggered by factors such as infections or autoimmune reactions. This immune response releases molecules that cause inflammation, resulting in tissue damage. Conditions …

Immunology · ImmunophenotypingCD Markers on B Cells: Development, Subsets and ImmunophenotypingB cells change their surface marker profile at every stage from bone marrow progenitor to antibody-secreting plasma cell, and those changes are what make each stage identifiable by flow cytometry. This guide sets out the markers stage by stage and subset by subset, explains the two that matter most in practice — CD19 and CD20 — and covers the gating strategy and controls that make the readout trustworthy.Browse B cell markers →CD19Pan-B, retained into plasma cellsCD20Lost on plasma cellsCD27The human memory B markerCD138Plasma cell identityKey takeawaysCD19 and CD20 are not interchangeable. CD19 ap …