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کیت غربالگری مهار کننده آنزیم آنژیوتانسین II (ACE2) آنزیم تبدیل آنژیوتانسین II(ACE2, EC 3.4.17.23)، کربوکسی پپتیداز بخشی از سیستم رنین-آنژیوتانسین است که کنترل فشار خون را با پاک کردن دیپپتید C- ترمینال آنژیوتانسین II کنترل می کند تا آن را به آنژیوتانسین 1-7 تبدیل کند. همچنین ، آنژیوتانسین I را تولید می کند تا عملکرد Ang 1-9 را با عملکرد ناشناخته تولید کند. ACE2 گیرنده انسدادی ویروسهای انسانی مانند SARS و HCoV-NL63 است. در سلولهای اندوتلیال عروقی قلب و کلیه بیان می شود. مهار کننده های ACE2 می توانند فشار خون را با تغییر نفوذپذیری عروقی تنظیم کنند. غربالگری برای مهار کننده های مولکول کوچک و پپتید نیز ممکن است در یافتن د …

Hepatocellular carcinoma (HCC) is one of the most frequently occurring cancers and there are 630,000 new cases diagnosed every year worldwide (De Minicis et al. 2013). HCC develops frequently from chronic hepatitis or cirrhosis of the liver (Thorgeirsson and Grisham 2002). These conditions result in hepatocyte destruction and promotion of immune cell invasion of the liver tissue, which in turn results in changes of the matrix and micro-environment of the liver (Bosch et al. 1999). Hepatitis B virus About 80% of HCC develops from hepatitis B virus (HBV), hepatitis C virus (HCV) and aflatoxin B1 (AFB) (Bosch et al. 1999). The development of HCC can take nea …

COVID-19 Rapid POC CE-IVDテスト（25テスト） 概要説明 Getein COVID-19 Rapid POC(臨床現場即時)キットは新型コロナウィルス(2019-nCoV)IgM/IgG抗体(金コロイド)検出用のワンステップテストであり、肺炎患者や疑わしい例から採取した血清、血漿、指先採血や全血サンプルにおける、2019新型コロナウィルスのIgMとIgG抗体を定量的に検出するものです。 要点 コロナウィルス感染症2019(COVID-19)は、かつてヒトで特定されたことのない新種のコロナウィルスである2019-nCoVによって引き起こされる伝染病です。この病気は主に、感染した人が咳やくしゃみをする際の飛沫を介して人々の間に広まります。暴露から症状が発現するまでの一般的な潜伏期間は2〜14日です。この病気は初期症状こそわずかか無症状の場合もありますが、やがて発熱や咳、息切れ、筋肉の痛みや倦怠感を呈します。さらに悪化すると肺炎や急性呼吸窮迫症候群も引き起こします。 ヒト血液中にある2019-nCoVのIgM・IgG抗体の検出は、COVID-19のスクリーニングの副次的手段として利用することが出来ます。 …

Stephen Reynolds, PhD Student, CERVIVA and Trinity College Dublin We hear almost daily now how everyone in their lifetime will be touched by cancer either directly or through a close family member. Cancer is our modern plague but even though it is so prevalent in modern society every cancer type is unique whether it is down to the type of cell that has become cancerous, it’s location in our body, mutations it’s acquired, was inherited or non-inherited and so forth. It is this lack of homogeneity that means there is no Silver Bullet for cancer treatment and the same is true for the testing and diagnosing cancers that arise in the population. The perfect screening pr …

By Charlotte O’Donnell PhD Cancer Carcinogenesis is a multi-step process that develops through epigenetic changes and mutation of multiple genes, including loss of function of tumour suppressor genes and gain of function of oncogenes. These genetic changes in normal cells can each contribute a growth advantage, leading to the transformation of cells into cancer cells [1]. In 2000, Hanahan and Weinberg described a series of 6 biological capabilities or ‘hallmarks’ acquired by most types of cancers during the multi-step development of cancer (Figure 1). They are as follows; ‘sustaining proliferative signalling’, …

Overview of Diabetes Mellitus Diabetes mellitus (DM) is a major epidemic that affects over 347 million people worldwide (Danaei et al. 2011). The World Health Organization predicts that the incidence of DM will rapidly increase with the number doubling by 2030 (Alwan et al. 2011; World Health Organization 2014). There are two major forms of DM, type 1 and type 2, which are clinically characterized by hyperglycaemia due to insulin insufficiency (Mathis et al. 2001). Type 1 diabetes (T1D), an autoimmune response resultant of genetic and environmental factors in which pancreatic β-cells within the islets of Langerhans are destroyed, comprises of appr …

Nanosilver is the most commercialised nanoparticle routinely used for its antibacterial purposes in food preservation, cosmetic and health products such as medical devices and wound treatment products (Vance et al., 2015). Interestingly, research on nanosilver has now progressed beyond antibacterial use to application as anticancer owing to some studies that have demonstrated the anticancer properties of nanosilver (Zhang et al., 2014, Zhang et al., 2016). Unfortunately, the wide antibacterial application of nanosilver coupled with the very high amounts of the nanoparticles in these commercialised and every day consumable products with the new anticancer application portends a seri …

By Charlotte O'Donnell PhD …

By Charlotte O'Donnell PhD A role for IL-33 in Barrier Function and Epithelial Wound Healing IL-33 is constitutively expressed by cells involved in the maintenance of mechanical barriers, including keratinocytes, lung and gut epithelial cells, fibroblasts and smooth muscle cells [1]. In these cells IL-33 is localized to the nucleus and mediates gene transcription. It is believed to maintain a quiescent state as it is only produced in barrier cells when they are senescent. Indeed, downregulation of IL-33 has been linked to the initiation of cellular proliferation in barrier cells. Once …

Danka Kozareva PhD Candidate, University College Cork The generation of neurons from stem cells The process of generating functional neurons from stem cells in the central nervous system (CNS) was believed to occur strictly during embryonic development and the early postnatal days in mammals (Ming and Song, 2011). A century ago this idea was challenged with the discovery of adult neurogenesis. Ezra Allen was the first to demonstrate that mitosis persisted in the lateral walls of adult albino rats (Allen, 1912). Several decades later Altman and Das (1965) followed up this research and determined that neurogenesis oc …

By Charlotte O'Donnell PhD The genes of the IL-36 family members are located in a cluster on chromosome 2 [1]. All three activating ligands, IL-36α, IL-36β and IL-36γ share the classic IL-1 β-trefoil structure, and lack a signal peptide. IL-36α, IL-36β, and IL-36γ lack a signal sequence, and thus cannot be transported to the endoplasmic reticulum. Similar to IL-1β and IL-18, the N-terminus must be cleaved for full agonist or antagonist bioactivity of each of the IL-36 cytokines. The protease responsible for this cleavage has not yet been elucidated. It is not thought to be a caspase as the site does not resemble a caspase cleavage site [2]. These th …

By Charlotte O’Donnell PhD Inflammation and Cancer The link between inflammation and cancer is well established, with inflammation highlighted as one of the enabling characteristics in cancer development [1]. As early as 1863, Virchow indicated that cancer tended to occur at sites of chronic inflammation. Indeed many cancers are associated with chronic inflammation. Patients with chronic hepatitis caused by Hepatitis B and C infection are at increased risk of developing hepatocellular carcinoma, while infection with human papilloma virus (HPV) is linked to the development of cervical cancer [2]. In the colon, infla …

Agata Miezaniec PhD Candidate, University of Nottingham, UK Epigenetics and the link to DNA Methylation The term ‘Epigenetics’ was not known in science until early 1940’s when British biologist Conrad Hal Waddington described his first developmental biology theories as an ‘epigenetic landscape’ (Waddington 1940). His hypothesis of genes controlled by epigenetics during embryo development started to evolve even before the discovery of DNA (Watson Crick 1953) and much before DNA methylation discovery (Miller et al 1978). Later on, the first definitions of the term ‘epigenetics’ tried to describe it as specific methyl …

PhD Summit - A Virtual Conference ELISA Genie is proud to announce the launch of a groundbreaking virtual conference - PhD Summit. This global virtual conference will take place the 18th & 19th of November 2020, starting at 2pm EDT. The goal of the conference is to provide an insight into the world of leading academics and their thoughts on success, strategy, failures, hiring, reading and work-life balance. Experience the conference wherever you are! Tune in live to listen to keynote speakers and watch sessions on demand. PhD Summit - Bringing Together Scientific Leaders …

SARS-CoV-2检测试剂盒──用于冠状病毒检测（2019-nCoV株）的一步式RT-qPCR试剂盒 用于可靠且可再生的一步式冠状病毒检测（2019-nCoV株） 产品概述： 一种目前被称为2019-nCoV或COVID-19的新型冠状病毒被宣布为在中国武汉市持续爆发的肺炎病例的病因。Assay Genie冠状病毒COVID-19（2019-nCoV株）一步式RT-qPCR检测试剂盒运用实时RT qPCR来进行呼吸道标本（痰；鼻咽、口咽抽吸物，洗液或拭子 ；气管抽吸物）中2019-nCoV或COVID-19的体外检测。 Assay Genie冠状病毒COVID-19（2019-nCoV株）一步式RT-qPCR检测试剂盒可在单个试管中进行高效cDNA合成和实时PCR。该试剂盒包含用于实时PCR的2x浓度的qPCR混合液。 qPCR混合液（2x）包含进行PCR反应所需的所有试剂（PCR引物、探针和模板除外）。此外，还包含RT混合物，其中包含RTase（逆转录酶）和RNase抑制剂的平衡混合物。 此外，该试剂盒还包括一套引物和荧光探针，以区分2019-nCoV和SARS-CoV（或蝙蝠衍生SARS中的CoV）。 冠状病毒2 …

  کیت تشخیص SARS-CoV-2 - کیت RT-qPCR یک مرحله ای برای تشخیص Coronavirus (Strain 2019-nCoV) برای تشخیص کوراو ویروس یک مرحله ای قوی و قابل تکرار (Strain 2019-nCoV) بررسی اجمالی محصولات Coronavirus رمان ، در حال حاضر با نام 2019-nCoV یا COVID-19 ، به عنوان عامل اتیولوژیک موارد شیوع ذات الریه پنومونی در ووهان ، چین اعلام شد. مجموعه Assay Genie Coronavirus COVID-19 (Strain 2019-nCoV) One-Step RT-qPCR Detection برای تشخیص آزمایشگاهی 2019-nCoV یا COVID-19 در نمونه های تنفسی (خلط ؛ نازوفارنکس ، آسپیراتهای o …

血管紧张素转换酶II（ACE2）活性测定试剂盒（荧光） 血管紧张素转换酶II（ACE2）活性测定试剂盒（荧光） 血管紧张素转化酶II（ACE2，EC 3.4.17.23）是一种基于锌的金属蛋白酶，为肾素-血管紧张素系统（RAS）的一部分，该系统通过裂解血管紧张素IIC端的二肽使之转变为血管紧张素1-7来控制血压调节。ACE2是人类冠状病毒（如SARS和HCoV-NL63）的受体。它在肺、肾和心脏的血管内皮细胞上表达。ACE2是心血管和冠状病毒诱发疾病的潜在治疗靶标。Assay Genie的ACE2活性测定试剂盒将有助于推进该领域的研究。该试剂盒利用活性ACE2的能力来裂解基于合成MCA的肽底物释放游离荧光团。释放的MCA可以使用荧光酶标仪轻松定量。我们也提供ACE2特异性抑制剂，可将ACE2活性与其他蛋白水解活性区分开来，该试剂盒的检测浓度低至0.4 mU。我们的测定试剂盒非常简单，并可以高通量使用。 window.__shgProductInits = window.__shgProductInits || new Array(); window.__shgProductCallbacks = window.__shgProductCallbacks || {}; …

کیت سنجش فعالیت آنزیم تبدیل آنژیوتانسین II (ACE2) (فلورومتریک) آنزیم مبدل آنژیوتانسین II ، (ACE2, EC 3.4.17.23) یک متالوپروتاز مبتنی بر روی است، بخشی از سیستم رنین-آنژیوتانسین (RAS) است که با پاک کردن دی پپتید ترمینال - Cآنژیوتانسین II ، تنظیم فشار خون را کنترل می کند. ACE2 گیرنده انسداد ویروسهای انسانی مانند SARS و HCoV-NL63 است. در سلولهای اندوتلیال عروقی ریه ، کلیه و قلب بیان می شود.ACE2 یک هدف درمانی بالقوه برای بیماری های قلبی عروقی و عروق کرونا ویروس است. مجموعه کیت سنجش فعالیت تست ژنتیکیACE2 به پیشرفت تحقیقات در این زمینه کمک خواهد کرد. این کیت از توانایی ACE2 فعال در برش بستر پپتید مصنوعی مبتنی بر MCA برای آ …

Descripción Para más información, por favor, contacte a hello@assaygenie.com El kit Assay Genie uso de investigación solamente COVID-19 Rapid POC (Point-of-Care) es una prueba rápida que detecta cualitativamente el coronavirus 2 del síndrome respiratorio agudo severo (SARS-CoV-2). El uso de una prueba rápida COVID-19 brinda la oportunidad de identificar a más personas que no saben que han sido infectadas. window.__shgProductInits = window.__shgProductInits || new Array(); window.__shgProductCallbacks = window. …

TEST RAPIDO POC COVID-19 Descrizione Il kit Assay Genie solo utilizzo per la ricerca. Il kit rapido COVID-19 POC (Point-of-Care) è un test rapido che rileva qualitativamente la sindrome respiratoria acuta grave coronavirus 2 (SARS-CoV-2). L'uso di un test rapido COVID-19 offre l'opportunità di identificare un numero maggiore di persone non consapevoli di essere state infettate. window.__shgProductInits = window.__shgProductInits || new Array(); window.__shgProductCallbacks = window.__shgProductCallbacks || {}; window.__shgProduct …

COVID-19 Schnelltest (Pflegepunkt) Beschreibung                       Das Assay Genie COVID-19 POC Schnelltest (Pflegepunkt) Kit nur für Forschungszwecke ist ein Schnelltest, der qualitativ das schwer akute respiratorische Syndrom Coronavirus 2 (SARS-CoV-2) nachweist. Die Verwendung eines COVID-19 POC Schnelltests bietet die Möglichkeit, mehr Personen zu identifizieren, die sich nicht bewusst sind, dass sie infiziert wurden. window.__shgProductInits = window.__shgProductInits || new Array …

Test COVID-19 rapide POC Description Le kit Assay Genie à utilisation seulement pour les recherches pour COVID-19 Rapid POC (point de soins) est un test rapide qui détecte qualitativement le coronavirus 2 du syndrome respiratoire aigu sévère (SARS-CoV-2). L'utilisation d'un test COVID-19 rapide permet d'identifier plus de personnes qui ne savent pas qu'elles ont été infectées. window.__shgProductInits = window.__shgProductInits || new Array(); window.__shgProductCallbacks = window.__shgP …

COVID-19 Testing Results in 15 Minutes Accurate detection of IgM and IgG patient-generated antibodies with the rapid, point-of-contact CE-IVD test window.SHOGUN_IMAGE_ELEMENTS = window.SHOGUN_IMAGE_ELEMENTS || new Array(); window.SHOGUN_IMAGE_ELEMENTS.push({ hoverImage: '', uuid: 's-7517deb9-2310-4b27-bd23-3eba3e3ba42c' }) COVID-19 Immunoassay Quick Facts > The COVID-19 Rapid Point-of-Contact CE-IVD Test Cassette is a qualitative, membrane-based immunoassay for the detection of Ig …

COVID-19（SARS-CoV-2）トリプレックスRT-qPCR検出キット COVID-19アッセイの概要： この製品は、新規コロナウイルスSARS-CoV-2によって引き起こされる疾患であるCOVID-19を検出するためのマルチプレックス蛍光プローブベースのRT-qPCRアッセイシステムです。 Taqman蛍光プローブは、レポーター-クエンチャーメカニズムに基づく特定のオリゴヌクレオチドです。 各プローブについて、5 '末端はフルオロフォアで標識され、3'末端はクエンチャーで標識されています。 プローブが無傷の場合、フルオロフォアが発する蛍光は消光剤に吸収され、蛍光シグナルは検出されません。 ただし、テンプレートの増幅中に、Taq DNAポリメラーゼの5'-3 'エキソヌクレアーゼ活性によりプローブが分解され、蛍光レポーターとクエンチャーが切断および分離された場合、蛍光シグナルを検出できます。 各分子単位複製配列の生成は、蛍光シグナルの生成を伴います。 PCRプロセス全体のリアルタイム監視は、蛍光シグナルの蓄積を監視することで評価できます。 この製品は、SARS-CoV-2の2つの独立した遺伝子と、標本の品質を評価するためにヒトRNAse P（RNP）遺伝子を標的とする内部コントロールを含む、単一のチューブでのトリプレックス検出を提供します。 特定の …