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By Charlotte O'Donnell PhD …

By Charlotte O'Donnell PhD A role for IL-33 in Barrier Function and Epithelial Wound Healing IL-33 is constitutively expressed by cells involved in the maintenance of mechanical barriers, including keratinocytes, lung and gut epithelial cells, fibroblasts and smooth muscle cells [1]. In these cells IL-33 is localized to the nucleus and mediates gene transcription. It is believed to maintain a quiescent state as it is only produced in barrier cells when they are senescent. Indeed, downregulation of IL-33 has been linked to the initiation of cellular proliferation in barrier cells. Once …

Danka Kozareva PhD Candidate, University College Cork The generation of neurons from stem cells The process of generating functional neurons from stem cells in the central nervous system (CNS) was believed to occur strictly during embryonic development and the early postnatal days in mammals (Ming and Song, 2011). A century ago this idea was challenged with the discovery of adult neurogenesis. Ezra Allen was the first to demonstrate that mitosis persisted in the lateral walls of adult albino rats (Allen, 1912). Several decades later Altman and Das (1965) followed up this research and determined that neurogenesis oc …

By Charlotte O'Donnell PhD The genes of the IL-36 family members are located in a cluster on chromosome 2 [1]. All three activating ligands, IL-36α, IL-36β and IL-36γ share the classic IL-1 β-trefoil structure, and lack a signal peptide. IL-36α, IL-36β, and IL-36γ lack a signal sequence, and thus cannot be transported to the endoplasmic reticulum. Similar to IL-1β and IL-18, the N-terminus must be cleaved for full agonist or antagonist bioactivity of each of the IL-36 cytokines. The protease responsible for this cleavage has not yet been elucidated. It is not thought to be a caspase as the site does not resemble a caspase cleavage site [2]. These th …

By Charlotte O’Donnell PhD Inflammation and Cancer The link between inflammation and cancer is well established, with inflammation highlighted as one of the enabling characteristics in cancer development [1]. As early as 1863, Virchow indicated that cancer tended to occur at sites of chronic inflammation. Indeed many cancers are associated with chronic inflammation. Patients with chronic hepatitis caused by Hepatitis B and C infection are at increased risk of developing hepatocellular carcinoma, while infection with human papilloma virus (HPV) is linked to the development of cervical cancer [2]. In the colon, infla …

Agata Miezaniec PhD Candidate, University of Nottingham, UK Epigenetics and the link to DNA Methylation The term ‘Epigenetics’ was not known in science until early 1940’s when British biologist Conrad Hal Waddington described his first developmental biology theories as an ‘epigenetic landscape’ (Waddington 1940). His hypothesis of genes controlled by epigenetics during embryo development started to evolve even before the discovery of DNA (Watson Crick 1953) and much before DNA methylation discovery (Miller et al 1978). Later on, the first definitions of the term ‘epigenetics’ tried to describe it as specific methyl …

PhD Summit - A Virtual Conference ELISA Genie is proud to announce the launch of a groundbreaking virtual conference - PhD Summit. This global virtual conference will take place the 18th & 19th of November 2020, starting at 2pm EDT. The goal of the conference is to provide an insight into the world of leading academics and their thoughts on success, strategy, failures, hiring, reading and work-life balance. Experience the conference wherever you are! Tune in live to listen to keynote speakers and watch sessions on demand. PhD Summit - Bringing Together Scientific Leaders …

SARS-CoV-2检测试剂盒──用于冠状病毒检测（2019-nCoV株）的一步式RT-qPCR试剂盒 用于可靠且可再生的一步式冠状病毒检测（2019-nCoV株） 产品概述： 一种目前被称为2019-nCoV或COVID-19的新型冠状病毒被宣布为在中国武汉市持续爆发的肺炎病例的病因。Assay Genie冠状病毒COVID-19（2019-nCoV株）一步式RT-qPCR检测试剂盒运用实时RT qPCR来进行呼吸道标本（痰；鼻咽、口咽抽吸物，洗液或拭子 ；气管抽吸物）中2019-nCoV或COVID-19的体外检测。 Assay Genie冠状病毒COVID-19（2019-nCoV株）一步式RT-qPCR检测试剂盒可在单个试管中进行高效cDNA合成和实时PCR。该试剂盒包含用于实时PCR的2x浓度的qPCR混合液。 qPCR混合液（2x）包含进行PCR反应所需的所有试剂（PCR引物、探针和模板除外）。此外，还包含RT混合物，其中包含RTase（逆转录酶）和RNase抑制剂的平衡混合物。 此外，该试剂盒还包括一套引物和荧光探针，以区分2019-nCoV和SARS-CoV（或蝙蝠衍生SARS中的CoV）。 冠状病毒2 …

  کیت تشخیص SARS-CoV-2 - کیت RT-qPCR یک مرحله ای برای تشخیص Coronavirus (Strain 2019-nCoV) برای تشخیص کوراو ویروس یک مرحله ای قوی و قابل تکرار (Strain 2019-nCoV) بررسی اجمالی محصولات Coronavirus رمان ، در حال حاضر با نام 2019-nCoV یا COVID-19 ، به عنوان عامل اتیولوژیک موارد شیوع ذات الریه پنومونی در ووهان ، چین اعلام شد. مجموعه Assay Genie Coronavirus COVID-19 (Strain 2019-nCoV) One-Step RT-qPCR Detection برای تشخیص آزمایشگاهی 2019-nCoV یا COVID-19 در نمونه های تنفسی (خلط ؛ نازوفارنکس ، آسپیراتهای o …

血管紧张素转换酶II（ACE2）活性测定试剂盒（荧光） 血管紧张素转换酶II（ACE2）活性测定试剂盒（荧光） 血管紧张素转化酶II（ACE2，EC 3.4.17.23）是一种基于锌的金属蛋白酶，为肾素-血管紧张素系统（RAS）的一部分，该系统通过裂解血管紧张素IIC端的二肽使之转变为血管紧张素1-7来控制血压调节。ACE2是人类冠状病毒（如SARS和HCoV-NL63）的受体。它在肺、肾和心脏的血管内皮细胞上表达。ACE2是心血管和冠状病毒诱发疾病的潜在治疗靶标。Assay Genie的ACE2活性测定试剂盒将有助于推进该领域的研究。该试剂盒利用活性ACE2的能力来裂解基于合成MCA的肽底物释放游离荧光团。释放的MCA可以使用荧光酶标仪轻松定量。我们也提供ACE2特异性抑制剂，可将ACE2活性与其他蛋白水解活性区分开来，该试剂盒的检测浓度低至0.4 mU。我们的测定试剂盒非常简单，并可以高通量使用。 window.__shgProductInits = window.__shgProductInits || new Array(); window.__shgProductCallbacks = window.__shgProductCallbacks || {}; …

کیت سنجش فعالیت آنزیم تبدیل آنژیوتانسین II (ACE2) (فلورومتریک) آنزیم مبدل آنژیوتانسین II ، (ACE2, EC 3.4.17.23) یک متالوپروتاز مبتنی بر روی است، بخشی از سیستم رنین-آنژیوتانسین (RAS) است که با پاک کردن دی پپتید ترمینال - Cآنژیوتانسین II ، تنظیم فشار خون را کنترل می کند. ACE2 گیرنده انسداد ویروسهای انسانی مانند SARS و HCoV-NL63 است. در سلولهای اندوتلیال عروقی ریه ، کلیه و قلب بیان می شود.ACE2 یک هدف درمانی بالقوه برای بیماری های قلبی عروقی و عروق کرونا ویروس است. مجموعه کیت سنجش فعالیت تست ژنتیکیACE2 به پیشرفت تحقیقات در این زمینه کمک خواهد کرد. این کیت از توانایی ACE2 فعال در برش بستر پپتید مصنوعی مبتنی بر MCA برای آ …

Descripción Para más información, por favor, contacte a hello@assaygenie.com El kit Assay Genie uso de investigación solamente COVID-19 Rapid POC (Point-of-Care) es una prueba rápida que detecta cualitativamente el coronavirus 2 del síndrome respiratorio agudo severo (SARS-CoV-2). El uso de una prueba rápida COVID-19 brinda la oportunidad de identificar a más personas que no saben que han sido infectadas. window.__shgProductInits = window.__shgProductInits || new Array(); window.__shgProductCallbacks = window. …

TEST RAPIDO POC COVID-19 Descrizione Il kit Assay Genie solo utilizzo per la ricerca. Il kit rapido COVID-19 POC (Point-of-Care) è un test rapido che rileva qualitativamente la sindrome respiratoria acuta grave coronavirus 2 (SARS-CoV-2). L'uso di un test rapido COVID-19 offre l'opportunità di identificare un numero maggiore di persone non consapevoli di essere state infettate. window.__shgProductInits = window.__shgProductInits || new Array(); window.__shgProductCallbacks = window.__shgProductCallbacks || {}; window.__shgProduct …

COVID-19 Schnelltest (Pflegepunkt) Beschreibung                       Das Assay Genie COVID-19 POC Schnelltest (Pflegepunkt) Kit nur für Forschungszwecke ist ein Schnelltest, der qualitativ das schwer akute respiratorische Syndrom Coronavirus 2 (SARS-CoV-2) nachweist. Die Verwendung eines COVID-19 POC Schnelltests bietet die Möglichkeit, mehr Personen zu identifizieren, die sich nicht bewusst sind, dass sie infiziert wurden. window.__shgProductInits = window.__shgProductInits || new Array …

Test COVID-19 rapide POC Description Le kit Assay Genie à utilisation seulement pour les recherches pour COVID-19 Rapid POC (point de soins) est un test rapide qui détecte qualitativement le coronavirus 2 du syndrome respiratoire aigu sévère (SARS-CoV-2). L'utilisation d'un test COVID-19 rapide permet d'identifier plus de personnes qui ne savent pas qu'elles ont été infectées. window.__shgProductInits = window.__shgProductInits || new Array(); window.__shgProductCallbacks = window.__shgP …

COVID-19 Testing Results in 15 Minutes Accurate detection of IgM and IgG patient-generated antibodies with the rapid, point-of-contact CE-IVD test window.SHOGUN_IMAGE_ELEMENTS = window.SHOGUN_IMAGE_ELEMENTS || new Array(); window.SHOGUN_IMAGE_ELEMENTS.push({ hoverImage: '', uuid: 's-7517deb9-2310-4b27-bd23-3eba3e3ba42c' }) COVID-19 Immunoassay Quick Facts > The COVID-19 Rapid Point-of-Contact CE-IVD Test Cassette is a qualitative, membrane-based immunoassay for the detection of Ig …

COVID-19（SARS-CoV-2）トリプレックスRT-qPCR検出キット COVID-19アッセイの概要： この製品は、新規コロナウイルスSARS-CoV-2によって引き起こされる疾患であるCOVID-19を検出するためのマルチプレックス蛍光プローブベースのRT-qPCRアッセイシステムです。 Taqman蛍光プローブは、レポーター-クエンチャーメカニズムに基づく特定のオリゴヌクレオチドです。 各プローブについて、5 '末端はフルオロフォアで標識され、3'末端はクエンチャーで標識されています。 プローブが無傷の場合、フルオロフォアが発する蛍光は消光剤に吸収され、蛍光シグナルは検出されません。 ただし、テンプレートの増幅中に、Taq DNAポリメラーゼの5'-3 'エキソヌクレアーゼ活性によりプローブが分解され、蛍光レポーターとクエンチャーが切断および分離された場合、蛍光シグナルを検出できます。 各分子単位複製配列の生成は、蛍光シグナルの生成を伴います。 PCRプロセス全体のリアルタイム監視は、蛍光シグナルの蓄積を監視することで評価できます。 この製品は、SARS-CoV-2の2つの独立した遺伝子と、標本の品質を評価するためにヒトRNAse P（RNP）遺伝子を標的とする内部コントロールを含む、単一のチューブでのトリプレックス検出を提供します。 特定の …

Interleukin 12 in COVID-19 Prophylactics Interleukin 12 is a cytokine with wide-reaching effects in the innate and adaptive immune system. Of considerable interest for intervention purposes is the ability of IL-12 to link innate resistance and adaptive immunity. Recently, IL-12’s unique ability to influence the production of interferon-γ and cause differentiation to Th1 cells has been harnessed to create a vaccine against COVID-19, the novel coronavirus pandemic caused by the SARS-CoV-2 virus. COVID-19 research tools ACE2 Activity …