NADP/NADPH Assay Kit (Colorimetric) (BA0092)
NADP/NADPH Assay Kit - InformationAssay Genie's NADP/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and is not interfered by NAD+/NADH. Our assay is a convenient method to measure NADP, NADPH and their ratio.
ApplicationsFor quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.
NADP/NADPH Assay Kit - Key Features
- Sensitive and accurate. Detection limit 0.1 , linearity up to 10 NADP+/NADPH in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 30 min at room temperature. No 37°C heater is required.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
NADP/NADPH Assay Kit - Data Sheet
|Kit Includes||Assay Buffer: 10 mL Glucose (1 M): 1.5 mL MTT Solution: 1.5 mL Enzyme Mix: 120 mL NADP Standard: 0.5 mL 1 mM NAD(P)/NAD(P)H Extraction Buffers: each 12 mL|
|Kit Requires||Pipetting (multi-channel) devices. Clear-bottom 96-well plates and plate reader|
|Method of Detection||OD565nm|
|Samples||Cell, tissue extracts etc|
|Protocol Length||30 min|
|Storage||Store all reagents at -20°C|
|Shelf Life||6 months|
More DetailsPyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable.