NADP/NADPH Assay Kit (Colorimetric) (BA0092)

NADP/NADPH Assay Kit - Information

Assay Genie's NADP/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP⁺/NADPH concentration in the sample. This assay is highly specific for NADP⁺/NADPH and is not interfered by NAD⁺/NADH. Our assay is a convenient method to measure NADP, NADPH and their ratio.

Applications

For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.

NADP/NADPH Assay Kit - Key Features

• Sensitive and accurate. Detection limit 0.1 , linearity up to 10 NADP+/NADPH in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 30 min at room temperature. No 37°C heater is required.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

NADP/NADPH Assay Kit - Data Sheet
Kit Includes	Assay Buffer: 10 mL Glucose (1 M): 1.5 mL MTT Solution: 1.5 mL Enzyme Mix: 120 mL NADP Standard: 0.5 mL 1 mM NAD(P)/NAD(P)H Extraction Buffers: each 12 mL
Kit Requires	Pipetting (multi-channel) devices. Clear-bottom 96-well plates and plate reader
Method of Detection	OD565nm
Detection Limit	0.1
Samples	Cell, tissue extracts etc
Species	All
Protocol Length	30 min
Size	100 tests
Storage	Store all reagents at -20°C
Shelf Life	6 months

More Details

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NADP⁺/NADPH concentration are very desirable.