NADP/NADPH Assay Kit (Colorimetric) (BA0092)

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SKU:
BA0092
€680

Description

ELISA Kit Technical ManualMSDS

NADP/NADPH Assay Kit - Information

Assay Genie's NADP/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and is not interfered by NAD+/NADH. Our assay is a convenient method to measure NADP, NADPH and their ratio.

Applications

For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.

NADP/NADPH Assay Kit - Key Features

  • Sensitive and accurate. Detection limit 0.1 , linearity up to 10 NADP+/NADPH in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 30 min at room temperature. No 37°C heater is required.
  • High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

NADP/NADPH Assay Kit - Data Sheet

Kit IncludesAssay Buffer: 10 mL Glucose (1 M): 1.5 mL MTT Solution: 1.5 mL Enzyme Mix: 120 mL NADP Standard: 0.5 mL 1 mM NAD(P)/NAD(P)H Extraction Buffers: each 12 mL
Kit RequiresPipetting (multi-channel) devices. Clear-bottom 96-well plates and plate reader
Method of DetectionOD565nm
Detection Limit0.1
SamplesCell, tissue extracts etc
SpeciesAll
Protocol Length30 min
Size100 tests
StorageStore all reagents at -20°C
Shelf Life6 months

More Details

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable.
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