Apoptosis & Necrosis Assays
Assay Genie have developed an extensive suite of assays to measure various forms of cell death including highly sensitive and reproducible detection of apoptosis & necrosis.
These assays detect key biomarkers such as caspase activity, DNA fragmentation and translocation of phosphatidylserine to the out surface of the plasma membrane.
Caspase 3/7 Activity Assay
Key Features
Quantify Caspase activity in various sample types
- Colorimetric & Fluorometric
- Use Caspase screening kits to identify Caspase inhibitors
- Read on a plate reader
TUNEL Assay Kit (Direct In Situ)
Key Features
Measure Apoptosis via DNA Fragmentation
- Fast Protocol: 3 hours
- Measure Apoptosis via DNA fragmentation
Caspase Assay Kits
Assay | Parameter | Time | Throughput | Instrument | Measurement | Size | Cat. No. |
Caspase 1 Activity in Cell Culture & Tissue Samples |
1-2 hours |
96 | 384 |
Plate Reader |
Absorbance: 405nm |
25 | 100 Assays |
BN00024 |
|
Caspase 2 Activity in Cell Culture & Tissue Samples |
1-2 hours |
96 | 384 |
Plate Reader |
Absorbance: 405nm |
25 | 100 Assays |
BN00048 |
|
Caspase 3/7 Activity in Cell Culture & Tissue Samples |
1-2 hours 1-2 hours |
96 | 384 |
Plate Reader |
Absorbance: 400nm |
25 | 100 Assays |
BN00013 |
|
Caspase 4 Activity in Cell Culture & Tissue Samples |
1-2 hours |
96 | 384 |
Plate Reader |
Absorbance: 400nm |
25 | 100 Assays |
BN00131 |
|
Caspase 5 Activity in Cell Culture & Tissue Samples |
1-2 hours |
96 | 384 |
Plate Reader |
Absorbance: 400nm |
25 | 100 Assays |
BN00075 |
|
Caspase 6 Activity in Cell Culture & Tissue Samples |
1-2 hours |
96 | 384 |
Plate Reader |
Absorbance: 400nm |
25 | 100 Assays |
BN00040 |
|
Caspase 8 Activity in Cell Culture & Tissue Samples |
1-2 hours |
96 | 384 |
Plate Reader |
Absorbance: 400nm |
25 | 100 Assays |
BN00032 |
|
Caspase 9 Activity in Cell Culture & Tissue Samples |
1-2 hours |
96 | 384 |
Plate Reader |
Absorbance: 400nm |
25 | 100 Assays |
BN00056 |
|
Caspase 10 Activity in Cell Culture & Tissue Samples |
1-2 hours |
96 | 384 |
Plate Reader |
Absorbance: 400nm |
25 | 100 Assays |
||
Caspase 12 Activity in Cell Culture & Tissue Samples |
1-2 hours |
96 | 384 |
Plate Reader |
Fluorescence: 400/505nm (Ex/Em) |
25 | 100 Assays |
BN00339 |
Key Antibodies
Annexin, Apoptosis & TUNEL Assays
Assay | Parameter | Time | Throughput | Instrument | Measurement | Size | |
Phosphatidylserine Detection |
15 minutes |
96 |
N/A |
Fluorescence: 488/530nm (Ex/Em) |
100 Assays |
||
Phosphatidylserine Detection |
15 minutes |
96 |
FACS, |
Fluorescence: 633/700nm (Ex/Em) |
100 Assays |
||
Phosphatidylserine Detection |
15 minutes |
96 |
FACS, |
N/A |
100 Assays |
||
Phosphatidylserine Detection |
15 minutes |
96 |
FACS, |
Fluorescence: 488/578nm (Ex/Em) |
100 Assays |
||
Phosphatidylserine Detection |
10 minutes |
96 |
FACS, |
Fluorescence: 543/570nm (Ex/Em) |
25 | 100 Assays |
BN00004 |
|
Phosphatidylserine Detection |
10 minutes |
96 |
FACS, |
Fluorescence: 649/670nm (Ex/Em) |
25 | 100 Assays |
BN00007 |
|
Phosphatidylserine Detection |
10 minutes |
96 |
FACS, |
Fluorescence: 488/530nm (Ex/Em) |
25 | 100 Assays |
BN00010 |
|
Phosphatidylserine Detection |
10 minutes |
96 |
FACS, |
Fluorescence: 488/670nm (Ex/Em) |
25 | 100 Assays |
BN00139 |
|
Apoptosis/Necrosis (FITC) Assay Kit |
1-Step | Live Cells |
10 minutes |
96 |
FACS, |
Fluorescence: 488/530nm (Ex/Em) |
25 | 100 Assays |
BN000473 |
1-Step | Live Cells |
10 minutes |
96 |
FACS, FACS, |
Fluorescence: |
25 | 100 Assays |
BN00476 |
|
1-Step | Live Cells |
10 minutes |
96 |
FACS, |
Fluorescence: |
25 | 100 Assays |
BN00479 |
|
DNA Fragmentation via Br-dUTP/anti-BrdU FITC Ab DNA Fragmentation via Br-dUTP/anti-BrdU Red Ab |
1 -3 hours |
96 |
FACS, |
Fluorescence: |
60 Assays |
||
DNA Fragmentation via Fluorescein-12-dUTP |
1 -3 hours |
96 |
FACS, |
Fluorescence: |
50 Assays |
||
DNA Fragmentation via Br-dUTP/anti-BrdU Biotin Ab |
1 -3 hours |
96 |
Microscopy |
IHC |
50 Assays |
||
DNA Fragmentation via Br-dUTP/anti-BrdU RED Ab |
1 -3 hours |
96 |
FACS, |
Fluorescence: |
60 Assays |