Apoptosis & Necrosis Assays

Assay Genie have developed an extensive suite of assays to measure various forms of cell death including highly sensitive and reproducible detection of apoptosis & necrosis.

These assays detect key biomarkers such as caspase activity, DNA fragmentation and translocation of phosphatidylserine to the out surface of the plasma membrane.

Caspase 3/7 Activity Assay

Key Features

Quantify Caspase activity in various sample types

Colorimetric & Fluorometric
Use Caspase screening kits to identify Caspase inhibitors
Read on a plate reader

TUNEL Assay Kit (Direct In Situ)

Key Features

Measure Apoptosis via DNA Fragmentation

Fast Protocol: 3 hours
Measure Apoptosis via DNA fragmentation

Caspase Assay Kits

Assay	Parameter	Time	Throughput	Instrument	Measurement	Size	Cat. No.
Capase 1 Assay Kit	Caspase 1 Activity in Cell Culture & Tissue Samples	1-2 hours	96 \| 384	Plate Reader	Absorbance: 405nm Fluorescence: 400/505nm (Ex/Em)	25 \| 100 Assays	BN00028 BN00029 BN00024 BN00025
Capase 2 Assay Kit	Caspase 2 Activity in Cell Culture & Tissue Samples	1-2 hours	96 \| 384	Plate Reader	Absorbance: 405nm Fluorescence: 400/505nm (Ex/Em)	25 \| 100 Assays	BN00052 BN00053 BN00048 BN00049
Capase 3/7 Assay Kit	Caspase 3/7 Activity in Cell Culture & Tissue Samples	1-2 hours 1-2 hours	96 \| 384	Plate Reader	Absorbance: 400nm Fluorescence: 400/505nm (Ex/Em)	25 \| 100 Assays	BN00017 BN00018 BN00013 BN00014
Capase 4 Assay Kit	Caspase 4 Activity in Cell Culture & Tissue Samples	1-2 hours	96 \| 384	Plate Reader	Absorbance: 400nm Fluorescence: 400/505nm (Ex/Em)	25 \| 100 Assays	BN00135 BN00136 BN00131 BN00132
Capase 5 Assay Kit	Caspase 5 Activity in Cell Culture & Tissue Samples	1-2 hours	96 \| 384	Plate Reader	Absorbance: 400nm Fluorescence: 400/505nm (Ex/Em)	25 \| 100 Assays	BN00084 BN00085 BN00075 BN00076
Capase 6 Assay Kit	Caspase 6 Activity in Cell Culture & Tissue Samples	1-2 hours	96 \| 384	Plate Reader	Absorbance: 400nm Fluorescence: 400/505nm (Ex/Em)	25 \| 100 Assays	BN00044 BN00045 BN00040 BN00041
Capase 8 Assay Kit	Caspase 8 Activity in Cell Culture & Tissue Samples	1-2 hours	96 \| 384	Plate Reader	Absorbance: 400nm Fluorescence: 400/505nm (Ex/Em)	25 \| 100 Assays	BN00036 BN00037 BN00032 BN00033
Capase 9 Assay Kit	Caspase 9 Activity in Cell Culture & Tissue Samples	1-2 hours	96 \| 384	Plate Reader	Absorbance: 400nm Fluorescence: 400/505nm (Ex/Em)	25 \| 100 Assays	BN00060 BN00061 BN00056 BN00057
Capase 10 Assay Kit	Caspase 10 Activity in Cell Culture & Tissue Samples	1-2 hours	96 \| 384	Plate Reader	Absorbance: 400nm Fluorescence: 400/505nm (Ex/Em)	25 \| 100 Assays	BN000123 BN000103
Capase 12 Assay Kit	Caspase 12 Activity in Cell Culture & Tissue Samples	1-2 hours	96 \| 384	Plate Reader	Fluorescence: 400/505nm (Ex/Em)	25 \| 100 Assays	BN00339 BN00340

Key Antibodies

Annexin, Apoptosis & TUNEL Assays

Assay	Parameter	Time	Throughput	Instrument	Measurement	Size
Annexin V-FITC Apoptosis Detection Kit	Phosphatidylserine Detection	15 minutes	96	N/A	Fluorescence: 488/530nm (Ex/Em)	100 Assays	CV0001
Annexin V-APC Apoptosis Detection Kit	Phosphatidylserine Detection	15 minutes	96	FACS, Fluorescent Microscopy	Fluorescence: 633/700nm (Ex/Em)	100 Assays	CV0002
Annexin V-Biotin Apoptosis Detection Kit	Phosphatidylserine Detection	15 minutes	96	FACS, Fluorescent Microscopy	N/A	100 Assays	CV0003
Annexin V-PE Apoptosis Detection Kit	Phosphatidylserine Detection	15 minutes	96	FACS, Fluorescent Microscopy	Fluorescence: 488/578nm (Ex/Em)	100 Assays	CV0004
Annexin V-CY3 Apoptosis Detection Kit	Phosphatidylserine Detection	10 minutes	96	FACS, Fluorescent Microscopy	Fluorescence: 543/570nm (Ex/Em)	25 \| 100 Assays	BN00004 BN00005
Annexin V-CY5 Apoptosis Detection Kit	Phosphatidylserine Detection	10 minutes	96	FACS, Fluorescent Microscopy	Fluorescence: 649/670nm (Ex/Em)	25 \| 100 Assays	BN00007 BN00008
Annexin V-GFP Apoptosis Detection Kit	Phosphatidylserine Detection	10 minutes	96	FACS, Fluorescent Microscopy	Fluorescence: 488/530nm (Ex/Em)	25 \| 100 Assays	BN00010 BN00011
Annexin V-PE/CY5 poptosis Detection Kit	Phosphatidylserine Detection	10 minutes	96	FACS, Fluorescent Microscopy	Fluorescence: 488/670nm (Ex/Em)	25 \| 100 Assays	BN00139 BN00140
Apoptosis/Necrosis (FITC) Assay Kit	1-Step \| Live Cells Apoptosis: Annexin V-FITC Necrosis: SYTOX Green	10 minutes	96	FACS, Fluorescent Microscopy	Fluorescence: 488/530nm (Ex/Em)	25 \| 100 Assays	BN000473 BN000474
Apoptosis/Necrosis (CY3) Assay Kit	1-Step \| Live Cells Apoptosis: Annexin V-CY3 Necrosis: SYTOX Green	10 minutes	96	FACS, Fluorescent Microscopy FACS, Fluorescent Microscopy	Fluorescence: SYTOX Green: 488/530nm (Ex/Em) Annexin V-CY3: 543/570nm (Ex/Em)	25 \| 100 Assays	BN00476 BN00477
Apoptosis/Necrosis (PE) Assay Kit	1-Step \| Live Cells Apoptosis: Annexin V-PE Necrosis: SYTOX Green	10 minutes	96	FACS, Fluorescent Microscopy	Fluorescence: SYTOX Green: 488/530nm (Ex/Em) Annexin V-PE: 548/578nm (Ex/Em)	25 \| 100 Assays	BN00479 BN00480
TUNEL Assay Kit (BrdU DNA)	DNA Fragmentation via Br-dUTP/anti-BrdU FITC Ab DNA Fragmentation via Br-dUTP/anti-BrdU Red Ab	1 -3 hours	96	FACS, Fluorescent Microscopy	Fluorescence: FITC: 488/520nm (Ex/Em) PI: 488/623nm (Ex/Em)	60 Assays	BN00654
TUNEL Assay Kit (Direct In Situ)	DNA Fragmentation via Fluorescein-12-dUTP	1 -3 hours	96	FACS, Fluorescent Microscopy	Fluorescence: FITC: 488/520nm (Ex/Em) PI: 488/623nm (Ex/Em)	50 Assays	BN00655
TUNEL Assay Kit (BrdU-IHC DNA)	DNA Fragmentation via Br-dUTP/anti-BrdU Biotin Ab	1 -3 hours	96	Microscopy	IHC	50 Assays	BN00656
TUNEL Assay Kit (BrdU-RED DNA)	DNA Fragmentation via Br-dUTP/anti-BrdU RED Ab	1 -3 hours	96	FACS, Fluorescent Microscopy	Fluorescence: BrdU: 488/576nm (Ex/Em) 7-AAD: 488/655nm (Ex/Em)	60 Assays	BN00657

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