Apoptosis & Necrosis Assays

Assay Genie have developed an extensive suite of assays to measure various forms of cell death including highly sensitive and reproducible detection of apoptosis & necrosis.

These assays detect key biomarkers such as caspase activity, DNA fragmentation and translocation of phosphatidylserine to the out surface of the plasma membrane.

Caspase 3/7 Activity Assay

Key Features

Quantify Caspase activity in various sample types

  • Colorimetric & Fluorometric
  • Use Caspase screening kits to identify Caspase inhibitors
  • Read on a plate reader

 

 

 


TUNEL Assay Kit (Direct In Situ)

Key Features

Measure Apoptosis via DNA Fragmentation

  • Fast Protocol: 3 hours
  • Measure Apoptosis via DNA fragmentation

Caspase Assay Kits

Assay Parameter Time Throughput Instrument Measurement Size Cat. No.

Capase 1 Assay Kit

Caspase 1 Activity in Cell Culture & Tissue Samples

1-2 hours

96 | 384

Plate Reader

Absorbance: 405nm
Fluorescence: 400/505nm (Ex/Em)

25 | 100 Assays

BN00028
BN00029

BN00024
BN00025

Capase 2 Assay Kit

Caspase 2 Activity in Cell Culture & Tissue Samples

1-2 hours

96 | 384

Plate Reader

Absorbance: 405nm
Fluorescence: 400/505nm (Ex/Em)

25 | 100 Assays

BN00052
BN00053

BN00048
BN00049

Capase 3/7 Assay Kit

Caspase 3/7 Activity in Cell Culture & Tissue Samples

1-2 hours

1-2 hours

96 | 384

Plate Reader

Absorbance: 400nm
Fluorescence: 400/505nm (Ex/Em)

25 | 100 Assays

BN00017
BN00018

BN00013
BN00014

Capase 4 Assay Kit

Caspase 4 Activity in Cell Culture & Tissue Samples

1-2 hours

96 | 384

Plate Reader

Absorbance: 400nm
Fluorescence: 400/505nm (Ex/Em)

25 | 100 Assays

BN00135
BN00136

BN00131
BN00132

Capase 5 Assay Kit

Caspase 5 Activity in Cell Culture & Tissue Samples

1-2 hours

96 | 384

Plate Reader

Absorbance: 400nm
Fluorescence: 400/505nm (Ex/Em)

25 | 100 Assays

BN00084
BN00085

BN00075
BN00076

Capase 6 Assay Kit

Caspase 6 Activity in Cell Culture & Tissue Samples

1-2 hours

96 | 384

Plate Reader

Absorbance: 400nm
Fluorescence: 400/505nm (Ex/Em)

25 | 100 Assays

BN00044
BN00045

BN00040
BN00041

Capase 8 Assay Kit

Caspase 8 Activity in Cell Culture & Tissue Samples

1-2 hours

96 | 384

Plate Reader

Absorbance: 400nm
Fluorescence: 400/505nm (Ex/Em)

25 | 100 Assays

BN00036
BN00037

BN00032
BN00033

Capase 9 Assay Kit

Caspase 9 Activity in Cell Culture & Tissue Samples

1-2 hours

96 | 384

Plate Reader

Absorbance: 400nm
Fluorescence: 400/505nm (Ex/Em)

25 | 100 Assays

BN00060
BN00061

BN00056
BN00057

Capase 10 Assay Kit

Caspase 10 Activity in Cell Culture & Tissue Samples

1-2 hours

96 | 384

Plate Reader

Absorbance: 400nm
Fluorescence: 400/505nm (Ex/Em)

25 | 100 Assays

BN000123
BN000103

Capase 12 Assay Kit

Caspase 12 Activity in Cell Culture & Tissue Samples

1-2 hours

96 | 384

Plate Reader

Fluorescence: 400/505nm (Ex/Em)

25 | 100 Assays

BN00339
BN00340

Key Antibodies

Annexin, Apoptosis & TUNEL Assays

Assay Parameter Time Throughput Instrument Measurement Size

Annexin V-FITC Apoptosis Detection Kit

Phosphatidylserine Detection

15 minutes

96

N/A

Fluorescence: 488/530nm (Ex/Em)

100 Assays

CV0001

Annexin V-APC Apoptosis Detection Kit

Phosphatidylserine Detection

15 minutes

96

FACS,
Fluorescent Microscopy

Fluorescence: 633/700nm (Ex/Em)

100 Assays

CV0002

Annexin V-Biotin Apoptosis Detection Kit

Phosphatidylserine Detection

15 minutes

96

FACS,
Fluorescent Microscopy

N/A

100 Assays

CV0003

Annexin V-PE Apoptosis Detection Kit

Phosphatidylserine Detection

15 minutes

96

FACS,
Fluorescent Microscopy

Fluorescence: 488/578nm (Ex/Em)

100 Assays

CV0004

Annexin V-CY3 Apoptosis Detection Kit

Phosphatidylserine Detection

10 minutes

96

FACS,
Fluorescent Microscopy

Fluorescence: 543/570nm (Ex/Em)

25 | 100 Assays

BN00004
BN00005

Annexin V-CY5 Apoptosis Detection Kit

Phosphatidylserine Detection

10 minutes

96

FACS,
Fluorescent Microscopy

Fluorescence: 649/670nm (Ex/Em)

25 | 100 Assays

BN00007
BN00008

Annexin V-GFP Apoptosis Detection Kit

Phosphatidylserine Detection

10 minutes

96

FACS,
Fluorescent Microscopy

Fluorescence: 488/530nm (Ex/Em)

25 | 100 Assays

BN00010
BN00011

Annexin V-PE/CY5 poptosis Detection Kit

Phosphatidylserine Detection

10 minutes

96

FACS,
Fluorescent Microscopy

Fluorescence: 488/670nm (Ex/Em)

25 | 100 Assays

BN00139
BN00140

Apoptosis/Necrosis (FITC) Assay Kit

1-Step | Live Cells
Apoptosis: Annexin V-FITC
Necrosis: SYTOX Green

10 minutes

96

FACS,
Fluorescent Microscopy

Fluorescence: 488/530nm (Ex/Em)

25 | 100 Assays

BN000473
BN000474

Apoptosis/Necrosis (CY3) Assay Kit

1-Step | Live Cells
Apoptosis: Annexin V-CY3
Necrosis: SYTOX Green

10 minutes

96

FACS,
Fluorescent Microscopy

FACS,
Fluorescent Microscopy

Fluorescence:
SYTOX Green: 488/530nm (Ex/Em)
Annexin V-CY3: 543/570nm (Ex/Em)

25 | 100 Assays

BN00476
BN00477

Apoptosis/Necrosis (PE) Assay Kit

1-Step | Live Cells
Apoptosis: Annexin V-PE
Necrosis: SYTOX Green

10 minutes

96

FACS,
Fluorescent Microscopy

Fluorescence:
SYTOX Green: 488/530nm (Ex/Em)
Annexin V-PE: 548/578nm (Ex/Em)

25 | 100 Assays

BN00479
BN00480

TUNEL Assay Kit (BrdU DNA)

DNA Fragmentation via Br-dUTP/anti-BrdU FITC Ab

DNA Fragmentation via Br-dUTP/anti-BrdU Red Ab

1 -3 hours

96

FACS,
Fluorescent Microscopy

Fluorescence:
FITC: 488/520nm (Ex/Em)
PI: 488/623nm (Ex/Em)

60 Assays

BN00654

TUNEL Assay Kit (Direct In Situ)

DNA Fragmentation via Fluorescein-12-dUTP

1 -3 hours

96

FACS,
Fluorescent Microscopy

Fluorescence:
FITC: 488/520nm (Ex/Em)
PI: 488/623nm (Ex/Em)

50 Assays

BN00655

TUNEL Assay Kit (BrdU-IHC DNA)

DNA Fragmentation via Br-dUTP/anti-BrdU Biotin Ab

1 -3 hours

96

Microscopy

IHC

50 Assays

BN00656

TUNEL Assay Kit (BrdU-RED DNA)

DNA Fragmentation via Br-dUTP/anti-BrdU RED Ab

1 -3 hours

96

FACS,
Fluorescent Microscopy

Fluorescence:
BrdU: 488/576nm (Ex/Em)
7-AAD: 488/655nm (Ex/Em)

60 Assays

BN00657

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