Caspase 3/7 Assay Kit (Fluorometric) (BA0020)
Caspase 3/7 Assay Kit - InformationAssay Genie's Caspase-3/7 activity Assay Kit provides a convenient means to measure caspase-3 activity in biological samples. In the assay, a specific substrate (N-Ac-DEVD-AFC) is cleaved by active caspase-3, forming a highly fluorescent product. The fluorescence intensity (Ex/Em = 400/490nm) is proportional to the caspase-3 activity.
ApplicationsFor quantitative determination of the apoptosis target caspase-3/7 activity and HTS screen for apoptosis inducers and inhibitors.
Caspase 3/7 Assay Kit - Key Features
- Safe. Non-radioactive assay.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Caspase 3/7 Assay Kit - Data Sheet
|Kit Includes||Assay Buffer: 12 mL Substrate: 240 mL DTT Solution: 240 mL|
|Kit Requires||Pipetting devices, centrifuge tubes, black flat bottom 96-well plates and plate reader.|
|Method of Detection||FL400/490nm|
|Samples||Cell and tissue lysate|
|Protocol Length||60 min|
|Storage||Store all reagents at -20°C|
|Shelf Life||12 months|
More DetailsCaspases are members of the aspartate-specific cysteinyl protease family that play a central role in apoptosis. Apoptosis is involved in a variety of physiological and pathological events, ranging from normal fetal development to diseases such as cancer, organ failure, and neurodegenerative diseases. Caspase-3 is key biomarker in the assessment of apoptosis and in understanding mechanism of apoptosis induction.
Aliases for CASP3 Gene
- Caspase 3
- Caspase 3, Apoptosis-Related Cysteine Peptidase
- Caspase 3, Apoptosis-Related Cysteine Protease
- SREBP Cleavage Activity 1
- Cysteine Protease CPP32
- Protein Yama
- EC 184.108.40.206
Entrez Gene Summary for CASP3 GeneThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]
UniProtKB/Swiss-Prot for CASP3 Gene CASP3_HUMAN,P42574Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a 216-Asp- -Gly-217 bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Protein details for CASP3 Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:P42574-CASP3_HUMAN
- Recommended name:Caspase-3
- Protein Accession:P42574
Protein attributes for CASP3 Gene
- Size:277 amino acids
- Molecular mass:31608 Da
- Quaternary structure:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce.