Pyruvate Assay Kit – Information
Assay Genie’s pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at Ex/Em = 530/585nm is directly proportional to pyruvate concentration in the sample.
For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.
Pyruvate Assay Kit – Key Features
- Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 2 to 500 μM (17 ug/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 μM for fluorimetric assays.
- Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays.
- Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
Pyruvate Assay Kit – Data Sheet
|Kit Includes||Enzyme Mix: 10 mL Dye Reagent: 120 mL Standard: 400 mL 25 mM Pyruvate|
|Kit Requires||Pipeting devices, centrifuge tubes, Clear flat-bottom 96-well plates, black 96-well or 384-well plates (e.g. Corning Costar) and plate reader|
|Method of Detection||OD570nm, or FL530/585nm|
|Detection Limit||OD, FL: 2, 0.2 μM|
|Protocol Length||20 min|
|Storage||Store all reagents at -20°C|
|Shelf Life||6 months|
Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery.