Acid Phosphatase Activity Fluorometric Assay Kit (BN00670)

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SKU:
BN00670
€461

Description

ELISA Kit Technical ManualMSDS

Acid Phosphatase Activity Fluorometric Assay Kit

Acid phosphatase (AP) dephosphorylates phosphate groups from the phosphate esters in acid conditions. Different forms of acid phosphatase are found in different organs, and their serum levels are used as a diagnosis for disease in the corresponding organs. For example, elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate resistant acid phosphatase levels may indicate the bone disease. Assay Genie's Acid Phosphatase Fluorometric Assay Kit uses non-fluorescent Methylumbelliferyl phosphate disodium (MUP) as the substrate which has Ex/Em=360/440 nm when dephosphorylated by AP. The kit is an ultra-sensitive, simple, direct and HTS-ready fluorometric assay designed to measure AP activity in serum and other bio-samples. The detection sensitivity is ~1 µU, more sensitive than colorimetric assays. The kit is suitable for both research and drug discovery.

Figure: AP 4-MU Standard Curve obtained using kit protocol.

Key Information Description

Product SKU

BN00670

Size

500 assays

Detection Method

Fluorescence (Ex/Em 360/440)

Species Reactivity

Mammalian

Applications

  • The detection sensitivity is ~1 µU, more sensitive than colorimetric assays.
  • The kit is suitable for both research and drug discovery.

Features and Benefits

  • Simple procedure; takes less than 1 hour
  • Fast and convenient
  • The kit is an ultra-sensitive, direct and HTS-ready fluorometric assay designed to measure AP activity in serum and other bio-samples.

Kit Components

  • AP Assay Buffer
  • MUP Substrate
  • AP Enzyme
  • Stop Solution

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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