Description
ADCC Assay Kit
Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Activation of immune system leads to removal of target cells infected by pathogens or transformed cells/cancer cells. This process is mediated by antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity. Assay Genie's Cell-Mediated Cytotoxicity Assay Kit contains carboxyfluorosuccinimide ester (CFSE), a green fluorescent probe that labels live target cells and 7-aminoactinomycin D (7-AAD), a red fluorescent probe that labels late apoptotic and necrotic target cells killed in the cytotoxicity assay. This assay does not require cell lysis and provides a direct measurement of cytotoxicity instead of indirect indicators such as release of ATP or lactate dehydrogenase activity. The flow cytometry-based method provides robust data and enables multi-parametric analysis.
Figure: Cytotoxicity Assay Kit: Jurkat cells (105 cells/ml) were grown in RPMI media supplemented with 10% FBS. Cells were treated with camptothecin (5 µM) overnight. Next day, cells were stained with CFSE and 7-AAD for 30 min. at 37°C. The graph (right side) displays the cytotoxic effect of the compound, illustrating apoptosis using CFSE and 7-AAD.
Key Information | Description |
Product SKU | BN00582 |
Size | 100 assays |
Measurement | Fluorescence (FL1 channel and FL3 channel) |
Kit Overview | Direct method to measure cell-mediated cytotoxicity. Does not require cell lysis. Provides multi-parametric analysis.
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Kit Contents |
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Storage | -20°C |
Shipping | Gel Pack |
Research Use | For Research Use Only! Not For Use in Humans. |