Adenosine Assay Kit (Fluorometric) (BN00594)

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SKU:
BN00594
  • Adenosine Assay Kit (Fluorometric) (BN00594)
  • Adenosine Assay Kit (Fluorometric) (BN00594)
  • Adenosine Assay Kit (Fluorometric) (BN00594)
€912

Description

ELISA Kit Technical ManualMSDS

Adenosine Assay Kit (Fluorometric)

Adenosine, a purine nucleoside, is present throughout the body. It plays an important role in energy transfer via the formation of ATP, ADP and AMP and in signal transduction via the formation of cAMP. Adenosine mediates its effects directly via adenosine receptors A1, A2A, A2B and A3. It regulates myocardial oxygen consumption and coronary blood flow, exerts anti-inflammatory effects throughout the body and also regulates the Renin-Angiotensin system. It also plays a role in tissue damage and repair, and cell death. Plasma adenosine levels are increased in patients with ischemic and non-ischemic heart failure. In Assay Genie's Adenosine Assay, adenosine is measured using adenosine deaminase followed by a multi-step enzymatic approach resulting in the generation of an intermediate that reacts with the Adenosine Probe with the formation of a fluorescent product. The fluorescent product is measured at Ex/Em = 535/587 nm. Detection range: 2-80 pmol.

Figure: (a).Adenosine Standard Curve (b). Measurement of Adenosine in pooled human plasma (20 µl) and human urine (4 µl of pre- treated urine, 2 times diluted during the pretreatment method) (c). Adenosine amount in human plasma and human urine. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00594

Size

100 recations

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Applications

Measurement of Adenosine in plasma and urine

Features and Benefits

  • Rapid & convenient
  • Detection range: 2-80 pmol

Kit Components

  • Adenosine Assay Buffer
  • Urine Clarifier
  • Adenosine Detector
  • Adenosine Convertor
  • Adenosine Developer
  • Adenosine Standard (10 mM)
  • Adenosine Probe (DMSO)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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