ADP Colorimetric Assay Kit II (BN00619)

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SKU:
BN00619
  • ADP Colorimetric Assay Kit II (BN00619)
  • ADP Colorimetric Assay Kit II (BN00619)
€655

Description

ELISA Kit Technical ManualMSDS

ADP Colorimetric Assay Kit II

ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. Dephosphorylation and rephosphorylation occur via various phosphorylases and kinases. ADP is stored in platelets and can be released to interact with variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. Assay Genie ADP Assay kit II is suitable for measuring ADP levels in samples that contain reducing substances, which may interfere with oxidase-based assays. In this assay, ADP in the presence of ADP Enzyme Mix is converted to an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450 nm. ADP Assay Kit II is simple, fast and high-throughput ready. It can detect less than 20 of ADP in samples.

Figure 1: (a) ADP Standards Curve (b) Measurements of ADP in fish liver (100µg), fish egg (200µg) & HeLa & Jurkat cells lysate (100µg) Assays were preformed following kit protocol.

Key Information Description

Product SKU

BN00619

Size

100 assays

Detection Method

Absorbance (450 nm)

Applications

The kit can detect less than 20 of ADP in samples.

Features and Benefits

  • Simple procedure; takes ~ 20 minutes
  • Convenient and sensitive
  • Kit contains all necessary reagents for accurate measurement of ADP levels

Kit Components

  • ADP Assay Buffer
  • ADP Enzyme Mix (Lyophilized)
  • Developer (Lyophilized)
  • Probe (Lyophilized)
  • ADP Standard (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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