Albumin (BCG) Assay Kit (Colorimetric) (BN00784)

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BN00784
  • Albumin (BCG) Assay Kit (Colorimetric) (BN00784)
  • Albumin (BCG) Assay Kit (Colorimetric) (BN00784)
  • Albumin (BCG) Assay Kit (Colorimetric) (BN00784)
€392

Description

ELISA Kit Technical ManualMSDS

Albumin (BCG) Assay Kit (Colorimetric)

Albumin is the most abundant protein in human blood and is highly conserved among vertebrates. It plays a pivotal physiological role in maintenance of plasma osmotic pressure, vascular permeability, and transport of cholesterol, bile pigments, nitric oxide, metals, and other small molecules in the body. It also functions as a free radical scavenger of reactive oxygen and nitrogen species, triggers cell signaling processes, possesses anti-inflammatory and coagulatory effects. Assay Genie's Albumin (BCG) Assay Kit is a simple high-throughput assay that detects Albumin concentration in serum. The assay is based on the selective interaction between Bromocresol Green (BCG) and Albumin forming a chromophore that can be detected at 620 nm. The signal is directly proportional to the amount of Albumin present in the serum. BCG does not react with other abundant plasma proteins like IgG. The assay can detect as low as 5 µg (0.01 g/dl) of albumin in serum samples.

Figure: (a) BSA Standard Curve (0-500 µg). (b) BSA Standard Curve (0-75 µg). (c) Albumin concentration in pooled human serum (PHS), fetal bovine serum (FBS) and bovine calf serum (BCS). Sample volumes (0-20 µl) were assayed following the kit protocol. Albumin concentration (g/dl): PHS: 3.8 ± 0.4; FBS: 1.6 ± 0.1; BCS: 2.6 ± 0.5.

Key Information Description

Product SKU

BN00784

Size

100 assays

Detection Method

Absorbance (620 nm)

Species Reactivity

Mammalian

Applications

Measurement of Albumin concentration in serum

Features and Benefits

  • Simple procedure,
  • High-throughput suitable
  • The assay can detect as low as 5 µg (0.01 g/dl) of albumin (BCG) in serum samples.

Kit Components

  • Albumin Assay Buffer
  • Bromocresol Green (BCG)
  • Bovine Serum Albumin (BSA, 50 mg/ml)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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