Assay Genie's Antibody-Biotin Labeling Kit provides an easy way to label antibodies with Biotin in a user-friendly spin column format. Due to its high affinity for tetrameric avidin proteins, biotin is ideal for labeling antibodies. Each pair of spin columns provided in the kit can be used to purify up to 1 mg of the labeled target antibody. The kit provides all of the reagents necessary to perform five labeling reactions using up to 1 mg of antibody per reaction. The biotin-labeled antibody can be directly used for multiple downstream applications including ELISA, western blot, Immunohistochemistry, Immunoassays, etc.

Figure: SEC chromatogram of an Anti-BSA IgG labeled with Biotin using a Superdex 200 HR 10/30 column at 0.5 ml/min. in 50 mM Tris and 0.25 M NaCl pH 7.5. The absorbance was monitored at 280 nm. The spin column format ensured that the purification of antibody was fast and there was no unreacted Biotin left after the antibody was purified according to the kit protocol.