Caspase-3 Colorimetric Assay Kit (BN00017)

SKU:
BN00017
€291

Description

ELISA Kit Technical ManualMSDS

Caspase-3 Colorimetric Assay Kit

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.

 

Figure: Induction of Caspase-6 Activity by Anti-Fas Antibody in Jurkat-T cells using caspase-3 colorimetric Assay Kit BN00017-18

Key Information Description

Detection Method

Absorbance (400 or 405 nm)

Size Options

  • 25 Assays
  • 100 Assays
  • 200 Assays
  • 400 Assays

Species Reactivity

Mammalian

Applications

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits

  • Simple one-step procedure; takes 1-2 hours
  • Fast and convenient
  • Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.

Kit Components

  1. Cell Lysis buffer
  2. 2X Reaction Buffer
  3. DEVD-pNA (4 mM)
  4. DTT (1 M)
  5. Dilution Buffer

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

Caspase-3 Assay Kit Citations

Anderson et al. Microbes exploit death-induced nutrient release by gut epithelial cells Nature 596(7871):262-267 (2021) PubMed: 34349263

Citations

Anderson et al. Microbes exploit death-induced nutrient release by gut epithelial cells Nature 596(7871):262-267 (2021) PubMed: 34349263
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Additional Information

Product type:
Cellular Assay
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