Caspase-4 Inhibitor Drug Screening Kit (Fluorometric) (BN00434)

SKU:
BN00434
€637

Description

ELISA Kit Technical ManualMSDS

Caspase-4 Inhibitor Drug Screening Kit (Fluorometric)

Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts. The Caspase-4 Inhibitor Drug Screening Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate LEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-4 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-4 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.

Key Information Description

Product SKU

BN00434

Size

100 assays

Detection Method

Fluorescence (Ex/Em 400/505 nm)

Species Reactivity

Mammalian

Applications

Effective means for screening caspase inhibitors using fluorometric methods.

Features and Benefits

  • Simple one-step procedure; takes only 1-1.5 hours
  • Fast and convenient
  • Inhibition of caspase-4 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.

Kit Components

  • 2X Reaction Buffer
  • Caspase Substrate LEVD-AFC (1 mM)
  • DTT (1 M)
  • Active Caspase-4 (Lyophilized)
  • Caspase Inhibitor, Z-VAD-FMK (2 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Cellular Assay
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