Caspase 9 Activity Assay (BN00056)

SKU:
BN00056
Product type:
Cellular Assay
Detection Method:
Fluorometric
€291

Description

Technical Manual MSDS

Caspase 9 Activity Assay

Assay Genies Caspase 9 Activity Assay provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (λmax = 400 nm). Upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-9 activity.

Caspase 9 Activity Assay Kit Data

Assay Information Description
Product Name: Caspase 9 Activity Assay
Product SKU: BN00056
Size:
  • 25 Assays
  • 100 Assays
  • 200 Assays
  • 400 Assays
Application:
  • Detect early/middle stages of apoptosis
  • Differentiate apoptosis from necrosis
Detection Method: Fluorometric (400 nm excitation filter and 505 nm emission filter)
Sample Type: Tissue Extracts & Cell Lysate
Storage and Stability: Store kit at –20°C (Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening). All reagents are stable for 1 year under proper storage conditions.
Shipping Conditions: Gel Pack
Usage: For research use only. Not for use in humans.

Caspase 9 Activity Assay Kit Components

Component Quantity
Cell Lysis Buffer 100 ml
2X Reaction Buffer 4 x 2 ml
LEHD-AFC (1 mM) 0.5 ml
DTT (1 M) 0.4 ml
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