Cathepsin G Activity Assay (BN00426)

SKU:
BN00426
Product type:
Cellular Assay
Detection Method:
Colorimetric
  • Cell Health Assays Cathepsin G Activity Assay BN00426
  • Cell Health Assays Cathepsin G Activity Assay BN00426
  • Cell Health Assays Cathepsin G Activity Assay BN00426
$800

Description

Technical Manual MSDS

Cathepsin G Activity Assay

Assay Genie’s Cathepsin G Activity Assay provides a quick and easy method for monitoring Cathepsin G. In the assay Cathepsin G cleaves the substrate to release the dye group pNA (4-Nitroaniline), which can be detect at 405 nm. In presence of the Cathepsin G specific inhibitor, the cleavage will be stopped.

Cathepsin G Activity Assay Kit Data

Assay Information Description
Product Name: Cathepsin G Activity Assay
Product SKU: BN00426
Size: 100 assays
Application: Detects alternative proteolytic enzymes such as the lysosomal cathepsin G proteases that can initiate or propagate proapoptotic signals.
Detection Method: Colorimetric (Absorbance 405 nm)
Sample Type: Serum, Tissue Extracts & Cell Lysate
Storage: Store the kit at -20°C, protect from light
Shipping Conditions: Gel Pack
Usage: For research use only. Not for use in humans.

Cathepsin G Assay Kit Components

Component Quantity
Assay Buffer 25 ml
Substrate 200 µl
Cathepsin G Inhibitor 20 µl
pNA Standard (0.1 M) 20 µl
Positive Control 1 vial

Product Images

Figure: a) Relative Activity Curve b) pNA Standard Curve c) Histogram of Positive Control with and without Inhibitor

Cathepsin G Background

Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. The protein is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.

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