Cell Fractionation Kit (BN00539)

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SKU:
BN00539
€631

Description

ELISA Kit Technical ManualMSDS

Cell Fractionation Kit

This Cell Fractionation system is designed to provide reproducible extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample. The method is fast and simple, needing only 2 hours and no ultracentrifugation involved. All four protein fractions obtained are suitable for many downstream applications such as 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.

Key Information Description

Product SKU

BN00539

Size

50 assays

Detection Method

Mammalian

Application

1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.

Features and Benefits

  • Simple procedure; takes less than 1 hour
  • Fast and convenient
  • The kit is designed for serial sample preparation of four distinct protein fractions including cytosol/particulate/cytoskeleton/nuclear fractions, from one sample.

Kit Components

  • Cytosol Extraction Buffer (CEB)
  • Membrane Extraction Buffer-A (MEB-A)
  • Membrane Extraction Buffer-B (MEB-B)
  • Nuclear Extraction Buffer (NEB)
  • DTT (1 M)
  • Protease Inhibitor Cocktail (lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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