Transformed cells can proliferate without attaching to surface Anchorage-independent cell growth is the hallmark of cell transformation. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). Assay Genie's Cell Transformation Assay is faster, stable, and more sensitive than the traditional soft-agar assay. The kit uses a quantitative dye that binds to nucleic acid and generates green fluorescence. This one-step method is non-radioactive and simple (just add-and-read, and does not require tedious labor such as counting colonies). The assay is high-throughput adaptable and has wide linear range from 50-60,000 cells. The entire assay can be finished within 7-8 days.

Figure: a) HeLa Cell Dose Curve; b) Anchorage-independent growth of 3T3 and HeLa cells. 3T3 and HeLa cells were serially diluted and seeded in agarose gel. Cells were solubilized and detected by the Quantitative Dye. c) Image of a HeLa Colony. HeLa cells were cultured for 7 days according to the kit protocol.