Endothelin Converting Enzyme 1 Activity Assay Kit (Fluorometric) (BN00767)

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SKU:
BN00767
  • Endothelin Converting Enzyme 1 Activity Assay Kit (Fluorometric) (BN00767)
  • Endothelin Converting Enzyme 1 Activity Assay Kit (Fluorometric) (BN00767)
$668.36

Description

ELISA Kit Technical ManualMSDS

Endothelin Converting Enzyme 1 Activity Assay Kit (Fluorometric)

Endothelin Converting Enzyme 1 (ECE1; EC: 3.4.24.71) is a membrane-bound metalloprotease that cleaves inactive big endothelin (big ET-1), which is the precursor of active endothelin. Studies have shown that big ET-1 could be used as a predictor of survival in patients suffering esophageal squamous cell carcinoma. ECE-1 is abundantly expressed in vivo in endothelial cells producing mature ET-1. As of today, Endothelin is the most potent vasoconstrictor known and it has cytokine- or hormone-like activities. Therefore, ECE-1 could play a significant role in pathogenesis of cardiovascular diseases and Alzheimer's Disease. Assay Genie's Endothelin Converting Enzyme 1 Activity Assay Kit utilizes the ability of active ECE-1 to cleave a synthetic substrate (MCA-based peptide) releasing free fluorophore, in the presence or absence of the ECE-1 Inhibitor Mix. The released f.luorophore can be easily quantified using a fluorescence microplate reader. Our kit uses a unique combination of substrate and inhibitor that specifically detects ECE-1 in a variety of biological samples. Contribution of other enzymes with similar catalytic properties - i.e. Endothelin Converting Enzyme 2 (ECE-2), Angiotensin-Converting Enzyme (ACE1, ACE2), and Neprilysin is compensated for within the assay. Our assay kit is simple, specific and can detect as low as 0.5 µU of ECE-1 activity.

Figure: (a) MCA Standard Curve. (b) Measurement of purified ECE-1 (5.5 ng) and ECE-2 (12 ng) activities. The kit can effectively discriminate ECE-1 activity from ECE-2 activity. (c) ECE-1 activity in Mouse Lung (1.1 µg protein) with or without Inhibitor Mix. (d) Measurement of ECE-1 Activity in Mouse Lung Extracts (1.1 µg protein), Mouse Heart Extracts (1.5 µg protein) and HeLa lysates (1.5 µg protein). All assays were performed following kit protocol.

Key Information Description

Product SKU

BN00767

Size

100 assays

Kit Summary

  • Detection method - Fluorescence (Ex/Em = 320/420 nm)
  • Applications- Detection of ECE1 activity in tissue/cell lysates/protein preparations

Detection Method

Fluorometric (Ex/Em = 320/420 nm)

Species Reactivity

Mammalian

Applications

Detection of ECE1 activity in tissue/cell lysates

Features and Benefits

  • Simple one-step reaction
  • Takes only 1-2 hrs
  • Non-radiometric fluorescent detection
  • HTP adaptable

Kit Components

  • ECE-1 Assay Buffer
  • ECE-1 (lyophilized)
  • ECE-1 Substrate (in DMSO)
  • ECE-1 Inhibitor Mix
  • MCA Standard (5 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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