GAPDH Activity Assay Kit (BN00904)

  • Metabolism Assays GAPDH Activity Assay Kit BN00904
  • Metabolism Assays GAPDH Activity Assay Kit BN00904
  • Metabolism Assays GAPDH Activity Assay Kit BN00904


ELISA Kit Technical ManualMSDS

GAPDH Activity Assay Kit

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH (housekeeping gene) expression is stable and constitutive. Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology. Assay Genie's GAPDH Activity Assay Kit provides a simple and sensitive method for monitoring GAPDH activity in various samples. In this assay, GAPDH catalyzes conversion of GAP into BPG and an intermediate, which reacts with a developer to form a colored product that absorbs maximally at 450 nm. Our high-throughput adaptable assay can detect GAPDH activity as low as 100 µU/mL in a variety of samples.

Figure 1: (a). NADH Standard Curve. (b). GAPDH activity in the Positive Control, 3T3 cells lysate and MDA-MB-468 cells lysate. (c). GAPDH specific activity calculated from 3T3 cell lysate (11.1 µg protein), and MDA-MB-468 cell lysate (2.76 µg protein). Assays were performed following kit protocol.

Key Information Description

Product SKU



100 assays

Detection Method

Absorbance (450 nm)


  • Measurement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity in various tissues/cells.
  • Analysis of glycolysis and pentose phosphate pathways

Features and Benefits

  • Simple, rapid & convenient.
  • Can measure GAPDH activityas low as 100 µU/mL in a variety of samples

Kit Components

  • GAPDH Assay Buffer
  • GAPDH Substrate
  • GAPDH Developer
  • NADH Standard (500 nmol)
  • GAPDH Positive Control

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

View AllClose

Additional Information

Product type:
Cellular Assay
View AllClose