GSH/GSSG Assay Kit (BN00532)

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SKU:
BN00532
€732

Description

ELISA Kit Technical ManualMSDS

GSH/GSSG Assay Kit

Glutathione is the major intracellular low-molecular-weight thiol that plays a critical role in cellular defense against oxidative stress in tissues and cells. Commercially available glutathione detection kits, such as the DTNB-enzyme cycling glutathione assay kit or the Monochlorobimane based assay kit hardly distinguish between reduced glutathione (GSH; FW: 307) and oxidized glutathione (GSSG; FW: 612). Assay Genie's Glutathione Detection Kit provides a unique, convenient tool for detecting GSH, GSSG, and total glutathione individually. In the assay, OPA, reacts with GSH (not GSSG), generating fluorescence, so GSH can be specifically quantified. Adding a reducing agent converts GSSG to GSH, so (GSH + GSSG) can be determined. To measure GSSG specifically, a GSH Quencher is added to remove GSH, preventing reaction with OPA (while GSSG is unaffected). Reducing agent is then added to destroy excess quencher and to convert GSSG to GSH. Thus, GSSG can be specifically quantified. The kit provides a unique procedure and buffer formula to eliminate protein thiol interference and to stabilize GSH and GSSG in solution.

Glutathione Standard Curve: Assay were prepared using standard GSH and GSSG following kit instructions. Diamond represents GSH standard curve. Circle represents GSSG standard curve produced by adding GSSG + GSH Quencher + Reducing Agent Mix. Triangle represents GSSG standard without adding Reducing Agent mix.

Key Information Description

Product SKU

BN00532

Size

100 assays

Detection Method

Fluorescence (Ex/Em 340/420 nm)

Species Reactivity

Mammalian

Applications

The kit provides unique formula and buffers and procedures for detecting the GSH, GSSG, and total glutathione individually

Features and Benefits

  • Simple procedure; takes only ~1-2 hours
  • Fast and convenient
  • The assay is easy to perform and detects 2-400 ng/µl of GSH, GSSG or total glutathione.

Kit Components

  • Glutathione Assay Buffer
  • PCA (Perchloric Acid, 6N)
  • KOH (<6N)
  • OPA Probe (o-phthalaldehyde )
  • Reducing Agent Mix
  • GSH Quencher
  • GSH Standard (FW: 307)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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