HAT Activity Fluorometric Assay Kit (BN00600)

(No reviews yet) Write a Review
SKU:
BN00600
  • HAT Activity Fluorometric Assay Kit (BN00600)
  • HAT Activity Fluorometric Assay Kit (BN00600)
  • HAT Activity Fluorometric Assay Kit (BN00600)
€690

Description

ELISA Kit Technical ManualMSDS

HAT Activity Fluorometric Assay Kit

Histone Acetyltransferases (HATs) are enzymes that acetylate histone substrates resulting in important regulatory effects on chromatin structure and assembly, and gene transcription. Modifications of these proteins by HATs play an important role in the control of gene expression, and their dysregulation has been linked to cancer, neurodegeneration, and other diseases. Assay Genie's HAT Activity Assay Kit utilizes Acetyl CoA and H3 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from Acetyl-CoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm. The assay can detect HAT activity as low as 6 mU in a variety of samples.

Figure: (a) Co A Standard Curve. (b) HAT Activity in HeLa Nuclear Extract. (c) Specific Activity of HeLa Nuclear Extract and purified recombinant pCAF. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00600

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Applications

  • Measurement of HAT activity in Nuclear Extracts
  • Measurement of HAT activity of purified enzyme preparations

Features and Benefits

  • Simple, rapid & convenient
  • The assay can detect HAT Activity less than 6 mU in a variety of samples

Kit Components

  • HAT Assay Buffer
  • Acetyl CoA (Lyophilized)
  • H3 Peptide (Lyophilized)
  • Substrate Mix (Lyophilized)
  • Developer
  • CoA Standard (Lyophilized)
  • Positive Control (HeLa Nuclear Extract)

Storage Conditions

-80°C

Shipping Conditions

Dry Ice

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose