HDAC3 Activity Fluorometric Assay Kit (BN00607)

SKU:
BN00607
Product type:
Cellular Assay
  • Cell Biology Assays HDAC3 Activity Fluorometric Assay Kit BN00607
  • Cell Biology Assays HDAC3 Activity Fluorometric Assay Kit BN00607
€581

Description

ELISA Kit Technical ManualMSDS

HDAC3 Activity Fluorometric Assay Kit

Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. HDACs are transcriptional repressors with crucial roles in mammalian development. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In Assay Genie's HDAC-3 activity Kit, HDAC-3 and Developer will deacetylate and cleave the substrate [R-H-K-K(Ac)-AFC] to release the quenched fluorescent group (AFC), which can be detected at Em/Ex = 380/500 nm. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput measurement of HDAC-3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC3 samples.

Figure: (a) AFC Standard Curve (b) Typical HDAC3 Activity Profile.

Key Information Description

Product SKU

BN00607

Size

100 assays

Detection Method

Fluorescence (Em/Ex = 380/500 nm)

Applications

Suitable for high throughput measurement of HDAC3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC-8 samples.

Features and Benefits

  • Simple procedure; takes ~ 60 min
  • Fast and convenient
  • Kit contains the necessary reagents for accurate measurement of HDAC3 activity

Kit Components

  • HDAC3 Assay Buffer
  • HDAC3 Substrate
  • HDAC3 Positive Control
  • AFC Standard (1 mM)
  • Developer
  • Trichostatin A

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.