HDL Uptake Assay Kit (Fluorometric) (BN00814)

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SKU:
BN00814
  • HDL Uptake Assay Kit (Fluorometric) (BN00814)
  • HDL Uptake Assay Kit (Fluorometric) (BN00814)
€976

Description

ELISA Kit Technical ManualMSDS

HDL Uptake Assay Kit (Fluorometric)

High-density lipoprotein (HDL) consists of a protein shell, containing apolipoproteins A-I and A-II, and a hydrophobic core consisting of cholesterol and triglycerides surrounded by phospholipids and cholesterol esters. HDL transports cholesterol to the liver or steroidogenic organs such as adrenals, ovary, and testes by both direct and indirect pathways. HDL-Cholesterol is removed by HDL receptors such as scavenger receptor BI (SR-BI), which mediate the selective uptake of cholesterol from HDL. Clinically, high levels of HDL has been associated with cardiovascular health. This is due to the ability of HDL particle to transport cholesterol from lipid-laden macrophages of atherosclerotic arteries to the liver for secretion into the bile by a process called as reverse cholesterol transport.

Assay Genie's HDL Uptake Assay Kit contains a fluorescently-labeled HDL that can be measured when taken up by cells. Unlabeled-HDL is included in the kit for assay validation.

Figure: (a) Fluorescently-labeled HDL Standard curve. (b) Fluorescently-labeled HDL uptake in HepG2 (hepatocytes) cells. Cells were starved for 4 hrs and incubated with Fluorescently-labeled HDL (10 µg) for 4 hrs. Fluorescently-labeled HDL (HDL) uptake was inhibited by SR-BI (HDL receptor) antibody (HDL + SRB1 Ab1), BLT1 (SR-BI Inhibitor; HDL + BLT1) and outcompeted by Unlabeled-HDL (HDL+ UL-HDL [20 µg]). Background was subtracted from the data (Wash off as described in note 1c, was subtracted as background). Assay was performed following the kit protocol.

Key Information Description

Product SKU

BN00814

Kit Size

100 assays

Detection

Fluorescence (Ex/Em 540/575 nm)

Application

  • Measure HDL uptake in different cell types
  • Screen, study or characterize stimulators or inhibitors of HDL uptake

Features

  • Only Kit on the market to quantitatively measure HDL uptake.
  • Includes unlabeled-HDL for assay validation.

Kit Contents

  • Assay Buffer Fluorescently-labeled HDL (5 mg/ml)
  • Unlabeled HDL (2x)

Storage

-20°C

Shipping

Gel Pack

Research Use

For Research Use Only! Not For Use in Humans.


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