Hexokinase (EC 1.1.1.49; also called HK, 6-Phosphate Glucose Kinase, ATP: D-Hexose 6-Phosphotransferase, ATP-dependent Hexokinase) is found in many organisms including bacteria, plants and mammals. Hexokinases play an important role in glucose metabolism. There are four isoforms (HK-I, II, III and IV). HK-I, HK-II, and HK-III have low Km, while HK-IV (also called Glucokinase) has 100-fold high Km for glucose. Hexokinase deficiency leads to diseases such as X-linked muscular dystrophy and rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis. Assay Genie's Hexokinase Activity Assay kit provides a quick and easy method for monitoring HK activity in a wide variety of samples. In this assay, HK converts glucose into glucose-6-Phosphate, which in turn undergoes a series of reactions and reduce to generate intense fluorescent product (Ex/Em = 535/587 nm). The assay is simple, sensitive and high-throughput adaptable and can detect as low as 2 µU of HK activity.

Unit Definition: One unit of Hexokinase is the amount of anzyme that egnerates 1.0µmol of NADPH per min at pH 8.0 at 25⁰C.

Figure: (a)NADPH Standard Curve (b) Kinetic measurement of HK Specific Activity in lysates prepared from orchid leaf (4µg) MCF-7 (0.56µg), Jurkat (0.65µg) and HeLa (0.62µg) cells. (c) HK Specific Activity of samples mentions in fig. b. Assays were preformed following Kit Protocol.