The HIPK1 Polyclonal Antibody (PAC057292) is a valuable tool for researchers studying the protein Homeodomain-interacting protein kinase 1 (HIPK1). This antibody, produced in rabbits, is highly specific to human samples and is ideal for use in Western blot applications. By binding to the HIPK1 protein, this antibody enables detection and analysis in various cell types, making it a crucial asset for research in areas such as cell signaling, apoptosis, and cancer biology.HIPK1 is a key player in cellular processes such as cell growth, differentiation, and stress response, making it a promising target for therapeutic interventions in diseases like cancer and neurodegenerative disorders.
By studying the function of HIPK1 with the help of this antibody, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic strategies. Overall, the HIPK1 Polyclonal Antibody is a reliable tool for researchers looking to delve deeper into the role of HIPK1 in various biological processes and diseases. Its specificity and sensitivity make it a valuable asset for studies aiming to further our understanding of HIPK1's function and potential as a therapeutic target.
IHC image of PACO57292 diluted at 1:200 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO57292 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO57292 diluted at 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation.
HIPK1: homeodomain interacting protein kinase 1 is a CMGC kinase of the DYRK family. May play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Expression elevated in many breast cancer cell lines. Null mice are resistant to carcinogens. Binds the p53 tumor suppressor. 4 alternatively spliced isoforms of the human protein have been described.Protein type: Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CMGC group; DYRK family; HIPK subfamilyChromosomal Location of Human Ortholog: 1p13.2Cellular Component: cytoplasm; nucleoplasm; nucleusMolecular Function: protein bindingBiological Process: eye development; positive regulation of angiogenesis
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms. [provided by RefSeq, Jul 2008]
