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Human and Mouse Phospho-FRS2 (Y436) and Total FRS2 PharmaGenie ELISA Kit (SBRS1836)

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SKU:
SBRS1836
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich
  • Human and Mouse Phospho-FRS2 Y436 and Total FRS2 PharmaGenie ELISA Kit SBRS1836
  • Human and Mouse Phospho-FRS2 Y436 and Total FRS2 PharmaGenie ELISA Kit SBRS1836
€1,469

Description

Human and Mouse Phospho-FRS2 (Y436) and Total FRS2 PharmaGenie ELISA Kit

Product SKU:SBRS1836
Size:96T
Application:The antibody pair provided in this kit recognizes Human and Mouse FRS2 phosphorylated at site Tyrosine-436 as well as total FRS2.
Uniprot:Q8WU20
Gene ID:10818
Pathway:MAPK Signaling
Synonyms:Fibroblast growth factor receptor substrate 2 (FGFR substrate 2) (FGFR-signaling adaptor SNT) (Suc1-associated neurotrophic factor target 1) (SNT-1)
Target Species:Human, Mouse
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Semi-Quantitative
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Anti-Phospho Antibody
  4. HRP-Conjugated Secondary Antibody
  5. Assay Diluent
  6. TMB One-Step Substrate
  7. Stop Solution
  8. Lysis Buffer
  9. Positive Control Sample

Other materials and equipment required:

The Assay Genie Human and Mouse Phospho-FRS2 (Y436) and Total FRS2 PharmaGenie ELISA Kit (SBRS1836) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 ul of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 ul of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Product ImageJurkat cells were treated or untreated with Pervanadate for 10 min at 37°C. Cell lysates were analyzed using this phosphoELISA:
Product ImageNIH3T3 cells were treated with Pervanadate. Solubilize cells at 4 x 10^7 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

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